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Changes in the abundance and structure of bacterial communities under long-term fertilization treatments in a peanut monocropping system

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Abstract

Background and aims

Peanut yield and quality are seriously compromised by continuous monoculturing in the red soil region of southern China. Monoculturing can cause soil degradation and an increase in soil-borne diseases. This research aimed to investigate the influence of long-term peanut monocropping and different fertilization treatments on peanut growth, soil physical and chemical properties and soil microbial community.

Methods

A long-term fertilization experiment established in 1996 was utilized to examine the effect of various fertilization treatments including chemical and organic fertilizers treatments. Deep 16S rRNA gene pyrosequencing highlighted changes in the abundance and structure of bacterial communities, especially of the pathogenic and beneficial bacterial communities in long term chemical fertilizer treatment in comparison to the organic manure treatment.

Results

Chemical fertilizer treatment causes a shift in bacterial community structure and decrease in diversity under the long-term monocropping in comparison to organic fertilizer. The abundance of the bacterial pathogen Ralstonia solanacearum, a causative agent of peanut wilt, was found to be associated with a loss of community diversity and loss of the peanut yield.

Conclusions

The organic fertilizers more effectively increase microbial diversity in the soil and changed the community structure. Long-term use of the chemical fertilizer leads to a decrease in microbial diversity of the soil and an increase in R. solanacearum with associated increase of peanut wilt. The potential decrease in diversity and competition between the bacterial community and the pathogen may be a contributing factor to increased disease during long-term chemical fertilizer use.

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Acknowledgments

We thank National Natural Science Foundation of China (41471236, 41325003) and Jiangsu Provincial Natural Science Foundation of China (BK2012891) for financial support.

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Authors and Affiliations

  1. Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China

    Wuxing Liu, Qingling Wang, Baozhan Wang, Ying Teng, Zhengao Li & Yongming Luo

  2. College of Environmental Sciences and Engineering, Yangzhou University, Yangzhou, 225009, China

    Xiaobing Wang

  3. Department of Microbiology, La Trobe University, Melbourne, VIC, 3086, Australia

    Ashley E. Franks

  4. Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China

    Yongming Luo

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  1. Wuxing Liu
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  2. Qingling Wang
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  3. Baozhan Wang
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  4. Xiaobing Wang
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  5. Ashley E. Franks
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  6. Ying Teng
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  7. Zhengao Li
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Correspondence to Wuxing Liu.

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Liu, W., Wang, Q., Wang, B. et al. Changes in the abundance and structure of bacterial communities under long-term fertilization treatments in a peanut monocropping system. Plant Soil 395, 415–427 (2015). https://doi.org/10.1007/s11104-015-2569-3

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