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INDETERMINATE DOMAIN PROTEIN binding sequences in the 5′-untranslated region and promoter of the SCARECROW gene play crucial and distinct roles in regulating SCARECROW expression in roots and leaves

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Abstract

SCARECROW (SCR) and SHORT-ROOT (SHR), which belong to the GRAS transcription factor family, are key regulators of root and leaf growth and development. Despite the importance of SCR expression for proper plant development, the mechanism of SCR regulation has not been clarified. A previous study showed that an INDETERMINATE DOMAIN transcription factor, JACKDAW (JKD), is essential for the expression of SCR in combination with SCR and SHR. In this study, we characterized possible binding sequences of INDETERMINATE DOMAIN PROTEIN in the 1.5 kb upstream region of SCR. Mutation in a binding sequence 340 bp upstream of the ATG increased transcriptional activation by JKD in transient assays using Arabidopsis cultured cells. However, the activity was not enhanced by SCR/SHR. Histochemical analysis of promoter activity in transgenic Arabidopsis plants carrying a fusion of the promoter and the β-glucronidase reporter gene showed that mutation of the −340 bp sequence eliminated most of the promoter activity, indicating that this sequence was indispensable for SCR expression. Promoter deletion of downstream sequences from −280 bp lost the enhanced activity by SCR/SHR in transient assays and activity in root tips and the bundle sheath (BS) in plants. Conversely, mutation at −480 bp did not significantly influence transcriptional activity in transient assays. However, most of SCR expression was lost except for the root tip in plants. The sequences around −1 kb appeared to regulate SCR expression negatively in plants. Together, these INDETERMINATE DOMAIN PROTEIN binding sequences have crucial and distinct functions in regulating SCR expression.

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Acknowledgements

We thank the RIKEN BioResource Center for Arabidopsis T87 suspension-cultured cells. We also thank Dr. Joseph Colasanti for critical reading of the manuscript and helpful comments. We also thank Dr. Yutaka Sato, Dr. Yuki Mizukami for technical support.

Author contributions

AK: study conception and design acquisition of data drafting of manuscript, SM: acquisition of data, AK: study conception and design analysis and interpretation of data drafting of manuscript.

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Authors and Affiliations

  1. Department of Biology, Shizuoka University, 836 Ohya Suruga-ku Shizuoka, 422-8529, Shizuoka-Shi, Japan

    Atsushi Kobayashi, Satoshi Miura & Akiko Kozaki

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  1. Atsushi Kobayashi
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  2. Satoshi Miura
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  3. Akiko Kozaki
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Correspondence to Akiko Kozaki.

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Kobayashi, A., Miura, S. & Kozaki, A. INDETERMINATE DOMAIN PROTEIN binding sequences in the 5′-untranslated region and promoter of the SCARECROW gene play crucial and distinct roles in regulating SCARECROW expression in roots and leaves. Plant Mol Biol 94, 1–13 (2017). https://doi.org/10.1007/s11103-016-0578-0

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  • DOI: https://doi.org/10.1007/s11103-016-0578-0

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