Abstract
We previously reported that rice plants expressing the chimeric receptor consisting of rice chitin oligosaccharides binding protein (CEBiP) and the intracellular protein kinase region of Xa21, which confers resistance to rice bacterial blight, showed enhanced cellular responses to a chitin elicitor N-acetylchitoheptaose and increased resistance to the rice blast fungus Magnaporthe oryzae. Here, we investigated whether CEBiP fused with another type of receptor-like protein kinase (RLK) also functions as a chimeric receptor. Fusion proteins CRPis consist of CEBiP and the intracellular protein kinase region of a true resistance gene Pi-d2. Transgenic rice expressing a CRPi showed enhanced cellular responses specifically to N-acetylchitoheptaose in cultured cells and increased levels of disease resistance against M. oryzae in plants. These responses depended on the amino acid sequences predicted to be essential for the protein kinase activity of CRPi. The structure of the transmembrane domain in CRPi affected the protein accumulation, cellular responses, and disease resistance in transgenic rice. These results suggest that the chimeric receptor consisting of CEBiP and Pi-d2 functions as a receptor for chitin oligosaccharides and CEBiP-based chimeric receptors fused with other RLKs may also act as functional receptors.
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Acknowledgments
We thank Drs. S. Mochizuki and S. Tanabe at the National Institute of Agrobiological Sciences as well as Dr. K. Kishimoto at the National Institute of Floricultural Science for their technical advice regarding M. oryzae inoculation and helpful discussions. We also thank K. Iwasaki, M. Kimura, H. Kurano, E. Nakajima, and K. Nakajima for producing rice transformants and daily assistance. This work was supported by the Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry in Japan.
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Kouzai, Y., Kaku, H., Shibuya, N. et al. Expression of the chimeric receptor between the chitin elicitor receptor CEBiP and the receptor-like protein kinase Pi-d2 leads to enhanced responses to the chitin elicitor and disease resistance against Magnaporthe oryzae in rice. Plant Mol Biol 81, 287–295 (2013). https://doi.org/10.1007/s11103-012-9998-7
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DOI: https://doi.org/10.1007/s11103-012-9998-7