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Photo-Degradation of Therapeutic Proteins: Mechanistic Aspects

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Abstract

Purpose: Therapeutic proteins are sensitive to photo-degradation by UV A and visible light. As none of the essential amino acids exhibits significant absorption in the UV A and visible light regions, the underlying mechanisms of photo-degradation induced by UV A and visible light are not well understood. This review addresses potential mechanisms, by which protein structure, oxidative modifications or impurities can promote the photo-degradation of therapeutic proteins during the exposure to UV A and visible light.

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Abbreviations

AGE:

Advanced glycation end products

CT:

Charge transfer

Di-Tyr:

Dityrosine

DOLD:

Deoxyglucason-lysine dimer

GOLD:

Glyoxal-lysine dimer

IgG1:

Immunoglobulin 1

Kyn:

Kynurenine

MOLD:

Methyl glyoxal-lysine dimer

NFK:

N-formylkynurenine

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ACKNOWLEDGMENTS AND DISCLOSURES

The author wishes to thank all graduate students, post-docs, and collaborators, who have contributed to our research in the field of protein photo-degradation. This research was funded, in part, by Genentech, the Genentech Foundation, and Amgen.

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  1. Department of Pharmaceutical Chemistry, The University of Kansas, 2093 Constant Avenue, Lawrence, Kansas, 66047, USA

    Christian Schöneich

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Schöneich, C. Photo-Degradation of Therapeutic Proteins: Mechanistic Aspects. Pharm Res 37, 45 (2020). https://doi.org/10.1007/s11095-020-2763-8

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