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The Relationship Between Taurine and 3-Nitrotyrosine Level of Hepatocytes in Experimental Endotoxemia

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Abstract

It has been proposed that taurine may function as an oxidant in a dose-dependent manner in vivo and in vitro. The present study was carried out to investigate the relationship between taurine concentration and 3-nitrotyrosine level, a stable marker of peroxynitrite action, in hepatocytes of guinea pig in endotoxemia before and after taurine administration. The levels of taurine and 3-nitrotyrosine were measured by HPLC method. In the present study, taurine was low concentration in hepatocytes exposed to endotoxemia. In taurine plus endotoxin treated animals, HPLC analysis showed higher taurine level compared with animals only supplemented with taurine. But 3-nitrotyrosine levels were same in both taurine alone and taurine plus endotoxin groups. In conclusion, taurine is able to prevent the damaging effect of peroxynitrite, at concentration measured in hepatocytes, in our experimental conditions.

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Authors and Affiliations

  1. Department of Biochemistry, Beytepe Military Hospital, Incek, Ankara, Turkey

    Hüsamettin Erdamar

  2. Department of Biochemistry, Faculty of Medicine, Gazi University, Ankara, Turkey

    Nurten Türközkan & Gonca Ozan

  3. Department of Biology, Faculty of Arts and Science, Gazi University, 06500, Teknikokullar, Ankara, Turkey

    Barboros Balabanli & Filiz Sezen Bircan

Authors
  1. Hüsamettin Erdamar
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  2. Nurten Türközkan
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  3. Barboros Balabanli
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  4. Gonca Ozan
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  5. Filiz Sezen Bircan
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Correspondence to Hüsamettin Erdamar.

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Erdamar, H., Türközkan, N., Balabanli, B. et al. The Relationship Between Taurine and 3-Nitrotyrosine Level of Hepatocytes in Experimental Endotoxemia. Neurochem Res 32, 1965–1968 (2007). https://doi.org/10.1007/s11064-007-9395-9

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  • DOI: https://doi.org/10.1007/s11064-007-9395-9

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