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Potential role of lncRNA-TSIX, miR-548-a-3p, and SOGA1 mRNA in the diagnosis of hepatocellular carcinoma

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A Correction to this article was published on 02 May 2019

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Abstract

Recent trends are moving towards the use of the circulating transcriptome as a potential diagnostic and therapeutic tool for hepatocellular carcinoma (HCC). The aim of this study is to identify circulatory RNA based biomarker panel, in addition to their relationship to the outcome in HCC. First, utilizing bioinformatics tools, we selected an HCC-specific RNA-based biomarker panel that depended on the integration of suppressor of glucose autophagy-associated (SOGA1) gene expression with the chosen panel of epigenetic regulators of this gene [long non-coding RNA antisense for X-inactive-specific transcript (lncRNA-TSIX) and microRNA-548-a-3p (miR-548-a-3p)]. Second, we attempted to validate these biomarkers using the sera of 65 patients with HCC, 34 patients with chronic hepatitis C virus (CHC) infection and 32 healthy volunteers. Finally, the expression levels of the chosen RNA-based biomarker panel were assessed in the serum samples using qRT-PCR assays. The panel of 3 RNA-based biomarkers (lncRNA-TSIX, miR-548-a-3p, and SOGA1) exhibited high sensitivity and specificity in differentiating HCC patients from CHC patients and healthy controls. Among these 3 RNAs, serum lncRNA-TSIX and SOGA1 were independent prognostic factor. The chosen circulatory RNA-based biomarker panel may serve as a diagnostic and prognostic biomarker for HCC.

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Change history

  • 02 May 2019

    The original publication has been updated. The family name of Alaa Habieb contained a typo that has now been corrected.

Abbreviations

ALT:

Alanine transaminase

AST:

Aspartate transaminase

AFP:

Alpha fetoprotein

CHC:

Chronic HCV

ceRNA:

Competing endogenous RNA

HCC:

Hepatocellular carcinoma

lncRNA-TSIX:

Long non-coding RNA antisense for X-inactive-specific transcript

miRNA, miR:

Micro ribonucleic acid

qRT-PCR:

Quantitative reverse-transcriptase polymerase chain reaction

SOGA1:

Suppressor of glucose autophagy-associated

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Acknowledgement

The manuscript was edited for English language usage, grammar, spelling and punctuation by one or more native English-speaking editors at Nature Research Editing Service (CERTIFICATE 8A4A-0338-9D25-FE77-368P). The author acknowledge dr Sara El-Nakeep; Clinical Tropical Medicine Department, Ain Shams University Hospital, for her effort in clinical samples provision.

Funding

This work was supported by the Egyptian Science and Technology Development Fund (RSTDG 2259).

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Authors and Affiliations

  1. Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, Abbassia, Cairo, P.O. Box 11381, Egypt

    Alaa Habieb, Marwa Matboli, Hanaa El-Tayeb & Farid El-Asmar

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  1. Alaa Habieb
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  2. Marwa Matboli
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  3. Hanaa El-Tayeb
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  4. Farid El-Asmar
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Correspondence to Marwa Matboli.

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Conflict of interest

Alaa Habib, Marwa Matboli, Hanaa El-Tayeb, and Farid El-Asmar declares that they have no competing interests.

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Written informed consent was obtained from all the participants in this study, which was performed in accordance with the Declaration of Helsinki and was approved by the Ethics Committee of Ain Shams Faculty of Medicine, Egypt (Ethical Approval Number; FN000023408).

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The original version of this article was revised: The family name of Alaa Habieb contained a typo that has now been corrected.

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Habieb, A., Matboli, M., El-Tayeb, H. et al. Potential role of lncRNA-TSIX, miR-548-a-3p, and SOGA1 mRNA in the diagnosis of hepatocellular carcinoma. Mol Biol Rep 46, 4581–4590 (2019). https://doi.org/10.1007/s11033-019-04810-x

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