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Effects of polymorphisms in the 3′ untranslated region of the porcine PPARGC1A gene on muscle fiber characteristics and meat quality traits

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Abstract

Peroxisome proliferator-activated receptor γ coactivator 1 α (PPARGC1A) is a transcriptional coactivator that is involved in a variety of biological processes including muscle fiber type composition. Here, we identified two single nucleotide polymorphisms (SNPs; *2690T>C and *2864T>C) and one insertion/deletion in the 3′ untranslated region of porcine PPARGC1A. These SNPs were genotyped by direct sequencing in a total of 439 pigs representing three different pig breeds (Berkshire, n = 156; Yorkshire, n = 163; Landrace, n = 120). We evaluated the effects of diplotypes of individual PPARGC1A 3′UTR SNPs on muscle fiber characteristics and meat quality traits. The *2690T>C polymorphism was significantly associated with the percentage of type I and IIb fibers for both muscle fiber number and area composition (P < 0.05), and also showed a significant association with muscle pH, a parameter of meat quality (P = 0.0188). The *2864T>C polymorphism was also associated with meat quality traits including muscle pH (P = 0.0071), drip loss (P = 0.0006), and lightness (P = 0.0702), but showed no significant association with muscle fiber characteristics. Interestingly, each SNP affected PPARGC1A expression significantly at the protein level but not at the mRNA level, thereby accounting for phenotypic variability among genotypes. Taken together, our data suggest that the *2690T>C and *2864T>C polymorphisms can be used as genetic markers for selection toward improved meat quality.

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Acknowledgments

This work was supported by a grant from the Next-Generation BioGreen 21 Program, Rural Development Administration, Republic of Korea, to Y.S.L (No. PJ0080892011), and by Brain Korea 21 Project from the Ministry of Education, Science, and Technology of Korea. This work was also supported by the Korea Research Foundation Grant funded by the Korean Government to K.C.H (2009-0076865).

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  1. College of Life Sciences and Biotechnology, Korea University, Seoul, 136-713, South Korea

    Jun-Seong Lee, Jun-Mo Kim, Jae-Sang Hong, Kyu-Sang Lim, Ki-Chang Hong & Young Sik Lee

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  1. Jun-Seong Lee
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Correspondence to Young Sik Lee.

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The authors Jun-Seong Lee, Jun-Mo Kim and Jae-Sang Hong contributed equally to this work.

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Lee, JS., Kim, JM., Hong, JS. et al. Effects of polymorphisms in the 3′ untranslated region of the porcine PPARGC1A gene on muscle fiber characteristics and meat quality traits. Mol Biol Rep 39, 3943–3950 (2012). https://doi.org/10.1007/s11033-011-1173-8

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