Abstract
SURF-6 is a bona fide nucleolar protein comprising an evolutionary conserved family that extends from human to yeast. The expression of the mammalian SURF-6 has been recently found to be regulated during the cell cycle. In order to determine the importance of SURF-6 in mammalian cells, we applied the Tet-On system to regulate conditionally, in response to tetracycline, the expression of an antisense RNA (asRNA) that targets Surf-6 mRNA in mouse NIH/3T3 cells. Induced Surf-6 asRNA caused an effective depletion of SURF-6 protein resulted in cell death and in an apparent arrest in the G1 phase of the cell cycle. These results provide for the first time evidence that expression of SURF-6 is essential for mammalian cell viability, and suggest that SURF-6 might participate in the progression of cell cycle.
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Abbreviations
- EGFP:
-
Enhanced green fluorescent protein
- PI:
-
Propidium iodide
- DAPI:
-
4′,6-Diamidino-2-phenylindole
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Acknowledgements
We are grateful to thank S.V. Khaidukov for flow cytometry. The pUHrT62-1 vector was a generous gift from Prof Wolfgang Hillen (University Erlangen, Germany). M. Polzikov has been supported by INTAS (Grant No. 03-55-0570). This work was supported by the Russian Foundation for Basic Research (Grant No. 04-04-48391).
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Polzikov, M., Magoulas, C. & Zatsepina, O. The nucleolar protein SURF-6 is essential for viability in mouse NIH/3T3 cells. Mol Biol Rep 34, 155–160 (2007). https://doi.org/10.1007/s11033-006-9028-4
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DOI: https://doi.org/10.1007/s11033-006-9028-4