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Quantitative proteomics to study the response of wheat to contrasting fertilisation regimes

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Abstract

Negative environmental impacts from mineral fertilisers and pesticides used in conventional cropping have raised concern over the sustainability of arable crop production. Organic cropping uses alternatives that avoid many of these negative environmental effects; however, crop yields can be significantly reduced, possibly due to a lower proportion of plant-available nutrients. To gain insights into the molecular effects of organic compared to conventional cropping systems on plant utilisation of nutrients, we used proteomics to analyse winter wheat (Triticum aestivum). Our aim was to investigate the effects of contrasting fertility management and crop protection regimes in organic and conventional cropping systems on the wheat flag leaf proteome and the association between the proteome and physiological traits. Wheat flag leaves were flash-frozen, lyophilised and milled prior to protein extraction (TCA/acetone) and analysed using 2D gel electrophoresis and MALDI-TOF MS. The abundance of 111 protein spots varied significantly between fertilisation regimes. Flag leaf N and P composition were significant drivers of differences in protein spot abundance, including major proteins involved in nitrogen remobilisation, photosynthesis, metabolism and stress response. These results indicate that molecular-based mechanisms are involved in the effect of contrasting cropping systems on nutrient utilisation and wheat grain yield. Using a functional genomics approach, we were able to identify proteins that are linked to causal genes, enabling the potential development of functional molecular markers for crop improvement in nutrient use efficiency.

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Acknowledgments

The authors gratefully acknowledge funding from the European Community financial participation under the Seventh Framework Program for Research, Technological Development and Demonstration Activities, for the Integrated Project NUE-CROPS FP7-CP-IP 222645.

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Authors and Affiliations

  1. School of Agriculture, Food and Rural Development, Molecular Agriculture Group, Nafferton Ecological Farming Group, Newcastle University, Nafferton Farm, Stocksfield, UK

    Catherine Tétard-Jones, Peter N. Shotton, Leonidas Rempelos, Julia Cooper, Mick Eyre & Carlo Leifert

  2. School of Applied Sciences, Northumbria University, Newcastle upon Tyne, NE1 8ST, UK

    Caroline H. Orr

  3. School of Biology, Molecular Agriculture Group, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK

    Angharad M. R. Gatehouse

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  1. Catherine Tétard-Jones
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  2. Peter N. Shotton
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  4. Julia Cooper
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  5. Mick Eyre
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  6. Caroline H. Orr
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  8. Angharad M. R. Gatehouse
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Correspondence to Angharad M. R. Gatehouse.

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Supplementary material 1 (DOCX 104 kb)

11032_2012_9796_MOESM2_ESM.tif

Online Resource 4: Values for ANOVA for grain yield across contrasting fertilisation and crop management regimes. Letters above the columns refer to Tukey test for significant differences between means (p < 0.05). (TIFF 995 kb)

11032_2012_9796_MOESM3_ESM.tif

Online Resource 8: Image of the DIGE reference gel (pH3–10 non-linear). The red arrows point to RuBisCo and glutamine synthetase protein spots that were up-regulated in flag leaves of compost- and mineral- (bold) fertilised wheat. The numbers on the right indicate the molecular mass. (TIFF 6616 kb)

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Tétard-Jones, C., Shotton, P.N., Rempelos, L. et al. Quantitative proteomics to study the response of wheat to contrasting fertilisation regimes. Mol Breeding 31, 379–393 (2013). https://doi.org/10.1007/s11032-012-9796-6

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