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Decreased expression of microRNA-21 is associated with increased cytokine production in peripheral blood mononuclear cells (PBMCs) of obese type 2 diabetic and non-diabetic subjects

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Abstract

The aim of this study was to investigate the role of miR-21 in inflammatory responses in peripheral blood mononuclear cells (PBMCs) of type 2 diabetic (T2D) and healthy subjects. 20 healthy and 20 T2D subjects were enrolled in the study. miR-21 expression in PBMCs of the subjects was measured using real-time PCR. IL-6 and TNF-α levels in culture supernatants were quantified using ELISA. miR-21 expression was not significantly different between the diabetic and nondiabetic groups. A downregulation of miR-21 expression was observed in PBMCs of obese subjects in both diabetic and nondiabetic groups. In addition, miR-21 expression was negatively correlated with weight, waist circumference, body mass index, and triglyceride in both the diabetic and nondiabetic groups. Our results also demonstrated that the PBMCs of obese subjects significantly secreted a higher level of IL-6 and TNF-α in comparison with the PBMCs of nonobese subjects. Furthermore, a significant inverse correlation between miR-21 expression and TNF-α and IL-6 production from the PBMCs was observed. These data suggest that miR-21 expression is decreased in PBMCs of obese subjects and reduced expression appears to be associated with increased secreted cytokine level in media of PBMCs of obese subjects.

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Abbreviations

BMI:

Body mass index

FBS:

Fasting blood sugar

IL-6:

Interleukin 6

IL-1β:

Interleukin 1β

IL-10:

Interleukin 10

IL-4:

Interleukin 4

IL-13:

Interleukin 13

LPS:

Lipopolysaccharide

miR-21:

MicroRNA-21

NF-κB:

Nuclear factor kappa B

PBMCs:

Peripheral blood mononuclear cells

TNF-α:

Tumor necrosis factor alpha

TLR-4:

Toll-like receptor-4

T2D:

Type 2 diabetes

WC:

Waist circumference

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Acknowledgments

We greatly appreciate the assistance provided by the staff of the Endocrinology and Metabolism Research Institute of Tehran University of Medical Sciences. We also thank all volunteers for their participation in the study. This work was financially supported by a grant (1391-01-97-1427) from the Diabetes Research Center, Endocrinology and Metabolism Research Institute of Tehran University of Medical Sciences.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran

    Hossein Mazloom, Samira Alizadeh & Reza Meshkani

  2. Diabetes Research Center, Endocrinology & Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran

    Ensieh Nasli Esfahani & Farideh Razi

  3. Endocrinology and Metabolism Research Center, Endocrinology & Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran

    Ensieh Nasli Esfahani & Farideh Razi

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  1. Hossein Mazloom
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Correspondence to Reza Meshkani.

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Mazloom, H., Alizadeh, S., Esfahani, E.N. et al. Decreased expression of microRNA-21 is associated with increased cytokine production in peripheral blood mononuclear cells (PBMCs) of obese type 2 diabetic and non-diabetic subjects. Mol Cell Biochem 419, 11–17 (2016). https://doi.org/10.1007/s11010-016-2743-9

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