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Collateral vessel growth induced by femoral artery ligature is impaired by denervation

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Abstract

Innervation plays an important role in development and remodeling of blood vessels. However, very little is known whether innervation is involved in arteriogenesis. In the present study, we tested the hypothesis that innervation may contribute to the process of arteriogenesis induced by ligature of femoral artery in rat/rabbit hind limb with or without denervation. We found that: (1) angiography showed more collateral vessels in the ligature side than that in ligature plus denervation side; (2) collateral vessels in denervation side was characterized by an inward remodeling; (3) in both collateral vessels (CVs) from only femoral ligature side as well as the ligature plus denervation side, ICAM-1 and VCAM-1 expression was up-regulated but increased VCAM-1 was more evident in the adventitia of collateral vessels of only femoral ligature side; (4) 7 days after surgery, in CVs from the femoral ligature side only, numerous macrophages (RAM11 positive cells) and high cell proliferation ratio (ki67 positive cells) were detected, but they were less in the denervation side. In conclusion, our data demonstrate for the first time that neural regulation is one of the factors that contributes to collateral vessel growth in rat/rabbit hind limb ischemic model by showing collateral vessel growth induced by femoral artery ligature is impaired by denervation.

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Acknowledgments

This work was supported by NSFC of Chinese government (No: 30771134 and No: 30971532).

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Authors and Affiliations

  1. Department of Histology & Embryology, Xiangya School of Medicine, Central South University, 172 Tong-Zhi-Po Road, Changsha, 410078, Hunan, People’s Republic of China

    Ming-ying Luo, Bao-lin Yang, Feng Ye, Song Peng, Wu Zhu, Hua Luo, Jian-Guo Wen & Wei-Jun Cai

  2. Department of Anatomy & Neurobiology, Xiangya School of Medicine, Central South University, Changsha, 410078, Hunan, People’s Republic of China

    Ming-ying Luo & Xiaoqiong Wu

  3. Department of Radiology, the 3rd Xiangya Hospital, Central South University, Changsha, 410078, Hunan, People’s Republic of China

    Song Peng, Bin Yi & Wei Wang

  4. Arteriogenesis Research Group, Max-Planck-Institute for Heart and Lung Research, 61231, Bad Nauheim, Germany

    Jutta Schaper & Wolfgang Schaper

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  1. Ming-ying Luo
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Correspondence to Wei-Jun Cai.

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Authors Ming-ying Luo, Bao-lin Yang, Feng Ye, Xiaoqiong Wu, and Song Peng contributed equally to this work.

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Luo, My., Yang, Bl., Ye, F. et al. Collateral vessel growth induced by femoral artery ligature is impaired by denervation. Mol Cell Biochem 354, 219–229 (2011). https://doi.org/10.1007/s11010-011-0821-6

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  • DOI: https://doi.org/10.1007/s11010-011-0821-6

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