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Radioanalytical methods for the measurement of melanin concentrating hormone (MCH) and detection its receptor in rat tissues

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Abstract

In the present paper the development and application of a novel melanin concentrating hormone radioimmunoassay and receptor-binding assay are described. 125I-labeling of melanin concentrating hormone (MCH) was performed by iodogen and the mono-iodinated peptide was separated by reversed-phase high performance liquid chromatography. Detection limit of the MCH specific assay was 0.2 fmol/ml. As a practical application of the novel radioimmunoassay, we measured the MCH concentration in different rat organs. High MCH concentrations were detected in the small intestine, pancreas, kidney, liver, trachea, hypothalamus and spinal cord. 125I-MCH was also suitable for RBA to demonstrate the presence of MCH receptors in the rat brain.

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Acknowledgments

This study was supported by the Hungarian National Office for Research and Technology (AGR-PIAC-13-1-2013-0008, TÁMOP-4.2.1.C-14/1/KONV-2015-0004 and TÁMOP-4.2.2.D-15/1KONV-2015-0016). A. Varga was supported by the Hungarian Brain Research program (KTIA_NAP_13-2-2014-0005) of the Hungarian Government.

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Authors and Affiliations

  1. Department of Pharmacology and Pharmacotherapy, University of Debrecen, Debrecen, 98 Nagyerdei krt, 4032, Hungary

    B. Lelesz, Z. Szilvássy, A. Varga, B. Juhász & J. Németh

  2. Department of Medical Chemistry, University of Szeged, Szeged, 8 Dóm tér, 6720, Hungary

    G. K. Tóth

  3. Institute of Cardiology, Division of Clinical Physiology, Faculty of Medicine, University of Debrecen, Debrecen, 22 Móricz Zsigmond krt, 4032, Hungary

    A. Tóth

  4. Department of Surgery, University of Debrecen, Debrecen, 22 Móricz Zsigmond krt, 4032, Hungary

    A. Enyedi

  5. Department of Pediatrics, University of Debrecen, Debrecen, 98 Nagyerdei krt, 4032, Hungary

    E. Felszeghy

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  1. B. Lelesz
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  2. Z. Szilvássy
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Correspondence to J. Németh.

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Lelesz, B., Szilvássy, Z., Tóth, G.K. et al. Radioanalytical methods for the measurement of melanin concentrating hormone (MCH) and detection its receptor in rat tissues. J Radioanal Nucl Chem 310, 1325–1333 (2016). https://doi.org/10.1007/s10967-016-4952-9

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