Abstract
In this present study, quorum quenching activity (QQ) of chitosan–selenium bionanocomposite with high biocompatibility was investigated. QQ activity was studied in Pseudomonas aeruginosa by measuring the expression level of Las B and RhII genes. Impact of gene expression on the major virulence factors production was also evaluated. Relative gene expression of both the genes was significantly reduced in all the dosages of CS–SeNC treatment, with subsequent reduction in the production of all the tested virulence factors. Biocompatibility of the nanocomposite was checked in zebrafish model which reveals that the administrated nanocomposite was not shown any undesirable effect on apoptosis, oxidative stress gene expression and liver histology. The present study implies that CS–SeNC prepared by in situ green science principles is a promising antibacterial agent against multiple drug-resistant bacterial strains, which exhibit an antagonistic effect by inhibiting the molecular mechanism of pathogenesis with noteworthy biocompatibility.
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We acknowledge SIST for the Characterisation of CS–SeNC.
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Karthick Raja Namasivayam, S., Nizar, M., Samrat, K. et al. Green Synthesis of Chitosan–Selenium Bionanocomposite with High Biocompatibility and Its Marked Impact on Las B and RhII Genes Expression in Pseudomonas aeruginosa. J Inorg Organomet Polym 32, 4186–4203 (2022). https://doi.org/10.1007/s10904-022-02431-9
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DOI: https://doi.org/10.1007/s10904-022-02431-9