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Investigation of Skin Barrier Functions and Allergic Sensitization in Patients with Hyper-IgE Syndrome

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Abstract

Purpose

Hyper-IgE syndrome (HIES) is a severe primary immunodeficiency, characterized by increased serum IgE levels as well as recurrent infections and atopic dermatitis (AD)-like skin lesions. AD is a chronic inflammatory skin disease with immunologic alterations (Th2-Th22 polarization) and characteristic skin barrier dysfunctions. Our aim was to investigate physicochemical skin barrier alterations and allergic sensitization in STAT3-HIES patients in order to explore whether skin barrier dysfunction can play a role in the eczematoid skin lesions in these patients.

Methods

In our experiments STAT3 and FLG mutation analyses were performed in STAT3-HIES (n = 7) and AD (n = 49) patients. Laboratory parameters (LDH and Eos counts), immunologic alterations (Th17 cell counts), allergic sensitization (total and specific IgE levels, skin prick tests, and medical history records), skin barrier changes [transepidermal water loss (TEWL), skin pH], serum and stratum corneum thymic stromal lymphopoietin (TSLP) levels were also examined.

Results

Impaired Th17 cell numbers, but normal physicochemical barrier functions, as well as serum and stratum corneum TSLP levels, were found in STAT3-HIES, while these parameters were significantly altered in AD patients. Allergic sensitization was detected in nearly all AD patients, while no signs of sensitization occurred in STAT3-HIES.

Conclusions

Our study demonstrated that the skin barrier functions of STAT3-HIES patients are not damaged and they differ significantly from the altered skin barrier functions of AD patients. A well-functioning physicochemical skin barrier may be one of the explanations on the contradiction between the extremely high total IgE levels and the lack of allergic sensitization in these patients. Our study underlines the importance of skin barrier in the development of allergic sensitization.

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Acknowledgments

This work was supported by the Hungarian Research Grants (OTKA K108421, TÁMOP-4.2.2.A-11/1/KONV-2012-0023-“DEFENSE-NET”, OTKA K101761, LP003/2011).

Conflict of Interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Division of Dermatological Allergology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98., Debrecen, 4032, Hungary

    Gábor Mócsai, Krisztián Gáspár, Zsolt Dajnoki & Andrea Szegedi

  2. Department of Dermatology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary

    Gábor Mócsai, Krisztián Gáspár, Zsolt Dajnoki & Andrea Szegedi

  3. Department of Infectious and Pediatric Immunology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary

    Beáta Tóth & László Maródi

  4. Institute of Laboratory Medicine, Faculty of Medicine, University of Debrecen, Debrecen, Hungary

    Edit Gyimesi

  5. DE-MTA “Lendület” Cellular Physiology Research Group, Department of Physiology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary

    Tamás Bíró

Authors
  1. Gábor Mócsai
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  2. Krisztián Gáspár
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Correspondence to Andrea Szegedi.

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Mócsai, G., Gáspár, K., Dajnoki, Z. et al. Investigation of Skin Barrier Functions and Allergic Sensitization in Patients with Hyper-IgE Syndrome. J Clin Immunol 35, 681–688 (2015). https://doi.org/10.1007/s10875-015-0200-2

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  • DOI: https://doi.org/10.1007/s10875-015-0200-2

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