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Resveratrol regulates the inflammation and oxidative stress of granulosa cells in PCOS via targeting TLR2

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Abstract

Polycystic ovary syndrome (PCOS) is featured as a common endocrine disorder in reproductive-aged women, while its pathophysiology is not fully illustrated. This study examined potential actions of resveratrol in PCOS cellular model and explored the underlying interaction between resveratrol and toll-like receptor 2 (TLR2). This study performed the bioinformatics analysis on two microarray datasets (GSE34526 and GSE138518). We found that TLR2 was one of potential hub genes that may be associated with PCOS. Further examination showed that TLR2 was highly expressed in granulosa cells from PCOS group compared with control. The in vitro studies showed that LPS intervention caused an increased expression of TLR2 and the pro-inflammatory mediators, and induced oxidative stress in the granulosa cells, which was concentration-dependently antagonized by resveratrol treatment. TLR2 silence significantly attenuated LPS-induced increase TNF-α, IL-1β, IL-6 and IL-8 expression and oxidative stress of granulosa cells. Furthermore, TLR2 overexpression promoted inflammatory response and oxidative stress in the granulosa cells, which was antagonized by resveratrol treatment. In conclusion, resveratrol could attenuate LPS-induced inflammation and oxidative stress in granulosa cells, and the underlying mechanisms may be related to the inhibitory effect of resveratrol on TLR2 expression in granulosa cells.

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Funding

This work was supported by Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University.

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Author notes

  1. Ben Yuan, Shuhong Luo and Liulian Feng equally contributed to this work.

Authors and Affiliations

  1. Department of Reproductive Medicine, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Edong Healthcare Group, Huangshi, 435000, Hubei, China

    Ben Yuan, Shuhong Luo, Liulian Feng, Junling Wang, Junbiao Mao & Bingbing Luo

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  1. Ben Yuan
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  2. Shuhong Luo
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  4. Junling Wang
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Contributions

BY, SL and LF conceived the study; BY, SL and LF performed the experiments; JW and JM performed the data analysis; BL prepared the graphs; all the authors approved the manuscript for submission.

Corresponding author

Correspondence to Ben Yuan.

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The authors declare no competing interests.

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All the experiments were approved by Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University.

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Supplementary file1 (PDF 71 KB) Supplemental Figure S1. PPI network of the common DEGs.

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Yuan, B., Luo, S., Feng, L. et al. Resveratrol regulates the inflammation and oxidative stress of granulosa cells in PCOS via targeting TLR2. J Bioenerg Biomembr 54, 191–201 (2022). https://doi.org/10.1007/s10863-022-09942-7

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  • DOI: https://doi.org/10.1007/s10863-022-09942-7

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