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In vitro comparison of three rifampicin loading methods in a reinforced porous β-tricalcium phosphate scaffold

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Abstract

The antibiotic compound, rifampicin (RFP), was loaded into porous reinforced β-tricalcium phosphate (β-TCP) scaffolds using three different solution adsorption methods. This resulted in drug delivery systems (DDS) generated by vacuum adsorption (VA), dynamic adsorption (DA), and static adsorption (SA). In vitro examination of the drug loading and release profiles of the DDS indicated that the unit mass of RFP loaded into the scaffold by the VA method (0.44 mg/g) was higher than that achieved by SA (0.42 mg/g) or DA (0.38 mg/g) (P < 0.05). The mechanical strength had no significant change after RFP-loading (P > 0.05). Moreover, there were no significant differences among the mechanical strength of three β-TCP DDS generated by loading RFP using SA, DA, and VA (P > 0.05). In vitro release testing showed an initial burst release of RFP from the three different DDS within the first 3 h and in the first 51 h, the cumulative release of RFP from VA-DDS, DA-DDS, and SA-DDS had reached 56.2, 83.6, and 88.6 %, respectively. Complete RFP release had occurred from VA-DDS, DA-DDS, and SA-DDS after 23, 17, and 15 days, respectively. As the VA-DDS method showed improved RFP loading and a more sustained drug release, this method is recommended for solution adsorption drug loading into porous β-TCP scaffolds to form a DDS.

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (81071453), the Program for Innovative Research Team of Shanghai Municipal Education Commission, the Fund for Interdisciplinary Research on Medicine and Engineering of Shanghai Jiaotong University (YG2011MS24), the Fund for Key Discipline of Shanghai Municipal Education Commission (J50206), and a Project fund administered by the Shanghai Science and Technology Committee (13DZ1940802, 11441900700).

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    Authors and Affiliations

    1. Shanghai Key Laboratory of Orthopedic Implant, Department of Orthopaedic Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200011, People’s Republic of China

      Junjie Yuan, Chen Han, Wei Sun, Dezhi Wang, Jie Zhao & Youzhuan Xie

    2. Department of Orthopedic Surgery, Shanghai Fengxian District Central Hospital, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital South Campus, Shanghai, 201499, People’s Republic of China

      Junjie Yuan

    3. Department of Otolaryngology, Head and Neck Surgery, Shanghai Jiao Tong University Affiliated Shanghai First People’s Hospital, Shanghai, 200080, People’s Republic of China

      Baoxin Wang

    4. Shanghai Bio-lu Biomaterials Co. Ltd., Shanghai, 201114, People’s Republic of China

      Xiao Lu & Jianxi Lu

    5. Department of Orthopedic Surgery, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200127, People’s Republic of China

      Chao Zhang

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    Junjie Yuan and Baoxin Wang have contributed equally to this work.

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    Yuan, J., Wang, B., Han, C. et al. In vitro comparison of three rifampicin loading methods in a reinforced porous β-tricalcium phosphate scaffold. J Mater Sci: Mater Med 26, 174 (2015). https://doi.org/10.1007/s10856-015-5437-z

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