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In vitro mineralization kinetics of poly(l-lactic acid)/hydroxyapatite nanocomposite material by attenuated total reflection Fourier transform infrared mapping coupled with principal component analysis

  • Biomaterials
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Abstract

Poly(l-lactic acid)/hydroxyapatite (PLLA/HA) nanocomposite, which combines the properties of PLLA and HA, is suitable to construct scaffold for bone tissue engineering. Its mineralization behavior plays a key role in composite’s property. In this present work, two PLLA/HA composites with porous and compact architecture were fabricated and soaked into simulated body fluid (SBF) at 37 °C for in vitro mineralization, respectively. An attenuated total reflection Fourier transform infrared (ATR FTIR) mapping coupled with principal component analysis was developed to investigate the mineralization kinetics. The FTIR images with an area of 300 × 300 μm2 were collected every 7 days. The results suggest that the mineralization of PLLA/HA composites in SBF follows a zero-order kinetic model, no matter what the architecture is. However, it follows a second-order model when the composite is degraded in phosphate-buffered saline solution based on our previous work. The mechanisms of the in vitro mineralization kinetics in different submersion solutions are discussed. Our results alert researchers that they should choose the mineralization medium cautiously.

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Authors and Affiliations

  1. State Key Laboratory of Chemical Resource Engineering, Analysis and Test Center, Beijing University of Chemical Technology, No. 15 Beisanhuan East Road, Chaoyang, Beijing, 100029, China

    Tongtong Dou, Nan Jing, Bingyao Zhou & Pudun Zhang

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  1. Tongtong Dou
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  2. Nan Jing
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  3. Bingyao Zhou
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  4. Pudun Zhang
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Correspondence to Pudun Zhang.

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Dou, T., Jing, N., Zhou, B. et al. In vitro mineralization kinetics of poly(l-lactic acid)/hydroxyapatite nanocomposite material by attenuated total reflection Fourier transform infrared mapping coupled with principal component analysis. J Mater Sci 53, 8009–8019 (2018). https://doi.org/10.1007/s10853-018-2169-8

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