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Involvement of single nucleotide polymorphisms in ovarian poor response

  • Genetics
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

Unpredictability in acquiring an adequate number of high-quality oocytes following ovarian stimulation is one of the major complications in controlled ovarian hyperstimulation (COH). Genetic predispositions of variations could alter the immunological profiles and consequently be implicated in the variability of ovarian response to the stimulation.

Design

Uncovering the influence of variations in AMHR2, LHCGR, MTHFR, PGR, and SERPINE1 genes with ovarian response to gonadotrophin stimulation in COH of infertile women.

Methods

Blood samples of the women with a good ovarian response (GOR) or with a poor ovarian response (POR) were collected. Genomic DNA was extracted, and gene variations were genotyped by TaqMan SNP Genotyping Assays using primer-probe sets or real-time PCR Kit.

Results

Except for PGR (rs10895068), allele distributions demonstrate that the majority of POR patients carried minor alleles of AMHR2 (rs2002555, G-allele), LHCGR (rs2293275, G-allele), MTHFR (rs1801131, C-allele, and rs1801133, T-allele), and SERPINE1 (rs1799889, 4G allele) genes compared to the GOR. Similarly, genotypes with a minor allele in AMHR2, LHCGR, MTHFR, and SERPINE1 genes had a higher prevalence among POR patients with the polymorphic genotypes. However, further genotype stratification indicated that the minor alleles of these genes are not associated with poor response. Multivariate logistic analysis of clinical−demographic factors and polymorphic genotypes demonstrated a correlation between FSH levels and polymorphic genotypes of SERPINE1 in poor response status.

Conclusions

Despite a higher prevalence of AMHR2, LHCGR, MTHFR, and SERPINE1 variations in the patients with poor ovarian response, it seems that these variations are not associated with the ovarian response.

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Acknowledgements

We would like to thank the medical and nursing staff of the Erfan, Laleh, and Taleghani hospitals in Tehran, Iran, for their helpful collaboration.

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Author notes

  1. Sayyed Mohammad Hossein Ghaderian and Reza Akbarzadeh contributed equally to this work.

Authors and Affiliations

  1. Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Sayyed Mohammad Hossein Ghaderian

  2. Institute of Anatomy, University of Lübeck, Lübeck, Germany

    Reza Akbarzadeh

  3. Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Reza Akbarzadeh

  4. Preventative Gynecology Research Center (PGRC), Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Saghar Salehpour

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  1. Sayyed Mohammad Hossein Ghaderian
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  2. Reza Akbarzadeh
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Correspondence to Saghar Salehpour.

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Ghaderian, S.M.H., Akbarzadeh, R. & Salehpour, S. Involvement of single nucleotide polymorphisms in ovarian poor response. J Assist Reprod Genet 38, 2405–2413 (2021). https://doi.org/10.1007/s10815-021-02242-w

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  • DOI: https://doi.org/10.1007/s10815-021-02242-w

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