Abstract
The dominant microalgal species, quantity of heterotrophic bacteria and Vibrio in the intestines and gills of Litopenaeus vannamei (Pacific white shrimp), positive detection rate of white spot syndrome virus (WSSV), and water quality indices were investigated at the final culture stage (88th day in culture season). Correlation of microalgal community, bacteria quantity, and shrimp production were analyzed by statistical analysis methods. Every 10 days, probiotics were used in groups A, B, and C, consisting of Bacillus, photosynthetic bacteria (PSB), and equal parts Bacillus and PSB, respectively. The results showed that production (25597.33 ± 928.46 kg ha−1) and survival rate (77.06 ± 9.00 %) was the highest in group C, but positive detection rate of WSSV was the lowest. The microalgal community of group C was significantly dominated by Chlorella pyrenoidosa, with an average density and dominance of (289.52 ± 142.10) × 107 cells L−1 and 0.878 ± 0.161, respectively. The correlation analysis indicated a significant negative correlation between Cyanophyta dominance and shrimp production (P < 0.05), while the relationship between production and Vibrio quantity was not significantly correlated (P > 0.05). Accordingly, microalgal dominant species should be controlled as a key factor in the shrimp culture season; in particular, the dominance of Cyanophyta should be restricted to a low level. Meanwhile, the combined use of Bacillus and PSB probiotics was considered an effective solution to optimize microalgal communities and controlling the cell density of Cyanophyta.
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Acknowledgments
This research was supported by the program of China agriculture research system (CARS-47), special fund for agro-scientific research in the public interest (201103034), science and technology planning project of Guangdong province (2012B020308003), projects of agricultural science and technology achievements transformation (2012GB23260551), and special scientific research funds for central non-profit institutes, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (2013ZD02).
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Cao, Yc., Wen, Gl., Li, Zj. et al. Effects of dominant microalgae species and bacterial quantity on shrimp production in the final culture season. J Appl Phycol 26, 1749–1757 (2014). https://doi.org/10.1007/s10811-013-0195-0
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DOI: https://doi.org/10.1007/s10811-013-0195-0