Skip to main content

Advertisement

Log in

Harvesting Chlorella minutissima using cell coagulants

  • Published:
Journal of Applied Phycology Aims and scope Submit manuscript

Abstract

Twelve salts were tested for their ability to coagulate microalgae cells in cultures of Chlorella minutissima. The final aim was to develop an easy and efficient approach for harvesting microalgae biomass in dense cultures. Aluminum, ferric, and zinc salts coagulated C. minutissima cultures, while optimum concentration was 0.75 and 0.5 g L−1 for sulfate and chloride salts, respectively. Aluminum salts were most efficient, but caused some cell lysis, which may render this approach inappropriate in some cases. Ferric and zinc salts were ranked second and third, respectively, according to their culture cell-coagulation efficiency. Ferric salts caused a change in the color of the cells, mainly at concentrations higher than 1 g L−1. Zinc salts were less harmful for the microalgal cells, but an additional problem was observed with cell aggregates adhering to the walls of the glass test tubes. Selection of the appropriate coagulant is related to the purpose of the coagulation process.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Bilanovic D, Shelef G, Sukenik A (1998) Flocculation of microalgae with cationic polymers: effects of medium salinity. Biomass 17:65–76

    Article  Google Scholar 

  • Buelna G, Bhattarai KK, de la Noue J, Taiganides EP (1990) Evaluation of various flocculants for the recovery of algal biomass growth on pig-waste. Biol Wastes 31:211–222

    Article  CAS  Google Scholar 

  • Gregory J, Duan J (2001) Hydrolyzing metal salts as coagulants. Pure Appl Chem 73:2017–2026

    Article  CAS  Google Scholar 

  • Gsordas A, Wang J-K (2004) An integrated photobioreactor and foam fractionation unit for the growth and harvest of Chaetoceros spp. in open systems. Aquacult Eng 30:15–30

    Article  Google Scholar 

  • Knuckey R, Brown M, Robert R, Frampton D (2006) Production of microalgal concentrates by flocculation and their assessment as aquaculture feeds. Aquacul Engin 35:300–313

    Article  Google Scholar 

  • Kotzabasis K, Hatziathanasiou A, Bengoa-Ruigomez MV, Kentouri M, Divanach P (1999) Methanol as alternative carbon source for quicker efficient production of the microalgae Chlorella minutissima: Role of the concentration and frequency of administration. J Biotechnol 70:357–362

    Article  CAS  Google Scholar 

  • Lebeau T, Robert JM (2003) Diatom cultivation and biotechnologically relevant products. Part I: cultivation at various scales. Appl Microbiol Biotechnol 60:612–623

    CAS  PubMed  Google Scholar 

  • Lee S, Kim S, Kim J, Kwon G, Yoon B, Oh H (1998) Effects of harvesting method and growth stage on the flocculation of the green alga Botryococcus braunii. Lett Apl Microbiol 27:14–28

    Article  Google Scholar 

  • Maeda K, Kuramochi H, Shinkawa T, Fukui K (2002) Solubility of two salts containing sulfate and chloride ions in water for ternary systems at 313 K. J Chem Eng Data 47:1472–1475

    Article  CAS  Google Scholar 

  • Makridis P, Vadstein O (1999) Food size selectivity of Artemia franciscana at three developmental stages. J Plankt Research 21:2191–2201

    Article  Google Scholar 

  • Millamena O, Aujero E, Borlongan I (1990) Techniques on algae harvesting and preservation for use in culture as larval food. Aquacult Eng 9:295–304

    Article  Google Scholar 

  • Oh H-M, Lee S, Park M-H, Kim H-S, Kim H-C, Yoon J-H, Kwon G-S, Yoon B-D (2001) Harvesting of Chlorella vulgaris using a bioflocculant from Paenibacillus sp. AM49. Biotechnol Lett 23:1229–1234

    Article  CAS  Google Scholar 

  • Poelman E, De Pauw N, Jeurissen B (1997) Potential of electrolytic flocculation for recovery of microalgae. Resour Conserv Recycl 19:1–10

    Article  Google Scholar 

  • Ponis E, Rombert R, Parisi G (2003) Nutritional value of fresh and concentrated algal diets for larval and juvenile Pacific oysters. Aquaculture 221:491–505

    Article  CAS  Google Scholar 

  • Price CA, Mendiola-Morgenthaler LR, Goldstein M, Breden EN, Guillard RRL (1974) Harvest of planktonic marine algae by centrifugation into gradients of silica in the CF-6 continuous-flow zonal rotor. Biol Bull 147:136–145

    Article  CAS  PubMed  Google Scholar 

  • Pushparaj B, Pelosi E, Torzillo G, Materassi R (1993) Microbial biomass recover using a synthetic cationic polymer. Biores Tech 43:59–62

    Article  CAS  Google Scholar 

  • Rossingol N, Vandajon L, Jaouen L, Quemeneur F (1999) Membrane technology for the continuous separation microalgae/culture medium: compared performances of cross-flow microfiltration and unfiltration. Aquaculture 20:191–208

    Article  Google Scholar 

  • Strasser B, Strasser R (1995) Measuring fast fluorescence transients to address environmental questions: The JIP-test. In: Mathis P (ed) Photosynthesis: from light to biosphere. Kluwer, Dordrecht, pp 977–980

    Google Scholar 

  • Widholm JM (1972) The use fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Technol 47:89–94

    Google Scholar 

  • Yu J, Tse S-W (1997) Flocculation of Pseudomonas with aluminum sulfate for enhanced biodegradation of synthetic dyes. Biotechnol Tech 11:479–482

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Aikaterini Papazi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Papazi, A., Makridis, P. & Divanach, P. Harvesting Chlorella minutissima using cell coagulants. J Appl Phycol 22, 349–355 (2010). https://doi.org/10.1007/s10811-009-9465-2

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10811-009-9465-2

Keywords

Navigation