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Association of NLRPs with pathogenesis of dry age-related macular degeneration

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Abstract

Background

Age-related macular degeneration (AMD) is the leading cause of blindness in the elderly population, and Dry AMD is the most common clinical subtype. However, effective measures for the early diagnosis and treatment of dry AMD have not been proposed. In recent years, NOD-like receptors (NLRs) have received attention in the study of AMD as an important class of pattern recognition receptors. We attempted to elucidate the pathogenesis of NLRs in dry AMD from the perspective of chronic inflammation.

Methods

This study involved 13 patients with dry AMD, 10 age- and sex-matched normal population without any history of disease and 8 patients with wet AMD as controls. Using RT-qPCR, the mRNA expression levels of NLRs in peripheral blood peripheral blood mononuclear cells (PBMCs) were compared to analyze the statistical differences in the expression contents among the three populations.

Results

The relative RNA expression of nucleotide-binding oligomerization-like receptor protein 12 (NLRP12) with negative regulation of inflammation was significantly lower in dry AMD patients than in normal people and wet AMD patients. And NLRX1, which also has an anti-inflammatory effect, was lower in dry AMD patients than in wet AMD patients. However, NLRP3 with proinflammatory effect was significantly expressed in wet AMD.

Conclusion

The significant decrease in NLRP12 in dry AMD may become a breakthrough in the study of dry AMD and systemic chronic inflammatory response. However, NLRP3 may have a more important role in wet AMD.

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Data availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

This work was supported by the National University Basic funding (Grant No. ZYGX2018J098), the Youth Scientific Research Fund of Sichuan Provincial People's Hospital (Grant No. 2017QN02), and the Scientific Research Project of Sichuan Provincial People's Hospital (Grant No. 2021kflx007), Projects of Sichuan Provincial Science and Technology Department (Grant No. 23ZDYF1942).

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Author notes

  1. Yiyun Zeng and Wentao Dong have contributed equally to this study.

  2. First correspondent of this article is Bolin Deng, and the cocorrespondent is Wanqiu Zhang.

Authors and Affiliations

  1. Department of Ophthalmology, Sichuan Provincial People’s Hospital, School of Medicine, University of Electronic Science and Technology of China, 32# W. Sec 2, 1St Ring Rd., Chengdu, 611731, Sichuan, China

    Yiyun Zeng, Wentao Dong, Wanqiu Zhang & Bolin Deng

Authors
  1. Yiyun Zeng
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  2. Wentao Dong
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Contributions

Conceptualization and methodology were contributed by W.Z. and B.D.; experiment processing was contributed by Y.Z., W.D. and W.Z.; formal analysis, data curation, and statistical analysis were contributed by Y.Z.; writing—original draft preparation, was contributed by Y.Z.; writing—review and editing, was contributed by Y.Z., W.D. and B.D.; supervision and project management were contributed by W.Z. and B.D. All authors have read and agreed to the published version of the manuscript.

Corresponding authors

Correspondence to Wanqiu Zhang or Bolin Deng.

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The authors declare that there is no conflict of interest associated with the manuscript.

Ethical approval

This study has been approved by the Ethical Committee of Sichuan Provincial People's Hospital. Samples were collected after people signed informed consent and samples were all collected from Sichuan Provincial People's Hospital.

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Zeng, Y., Dong, W., Zhang, W. et al. Association of NLRPs with pathogenesis of dry age-related macular degeneration. Int Ophthalmol 43, 4869–4878 (2023). https://doi.org/10.1007/s10792-023-02889-7

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  • DOI: https://doi.org/10.1007/s10792-023-02889-7

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