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Auraptene Mitigates Colitis Induced by Dextran Sulfate Sodium in Mice by Regulating Specific Intestinal Flora and Repairing the Intestinal Barrier

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Abstract

Auraptene (AUT) is widely known to possess both antioxidant and anti-inflammatory properties. This study attempted to evaluate the protective effects of AUT in dextran sodium sulfate (DSS)-induced colitis in mice and to determine the underlying molecular mechanisms. Our results suggest that AUT substantially minimizes the severity and worsening of DSS-induced colitis in mice, indicated by the lengthening of the colon, lower disease activity index, reduced oxidation levels, and attenuated inflammatory factors. Molecular studies revealed that AUT reduces the nuclear translocation of nuclear factor-κB (NF-κB), thereby inhibiting the expression of inflammatory factors. Additionally, AUT promotes the diversity of the intestinal flora in mice with colitis by increasing the number of beneficial bacteria such as Lactobacillaceae and lowering the number of harmful bacteria. In conclusion, AUT mitigates DSS-induced colitis by maintaining the integrity of the intestinal barrier and modulating the levels of the intestinal microbial species.

Graphical Abstract

TOC Graphic. Diagram summarizing the study findings. Auraptene mitigates dextran sulfate sodium-induced colitis in mice by regulating specific intestinal flora and repairing the intestinal barrier.

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Data availability

The data supporting the findings in this study will be made available upon reasonable request to the corresponding authors.

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Funding

This study was supported by the Norman Bethune Program of Jilin University (Grant No. 2022B15) and the Science and Technology Development Program of Jilin Province (Grant Nos. YDZJ202301ZYTS103 and YDZJ202201ZYTS002).

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Authors and Affiliations

  1. Department of Gastrointestinal Colorectal Surgery, China-Japan Union Hospital of Jilin University, Changchun, 130033, China

    Tong Chen, Naizhong Jin, Qi Zhang, Zhongming Li, Qiutao Wang & Xuedong Fang

Authors
  1. Tong Chen
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  2. Naizhong Jin
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  3. Qi Zhang
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  4. Zhongming Li
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  5. Qiutao Wang
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  6. Xuedong Fang
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Contributions

Conceptualization: Xuedong Fang, Tong Chen, Naizhong Jin, Zhongming Li, and Qiutao Wang. Methodology: Qiutao and Tong Chen. Formal analysis: Xuedong Fang and Naizhong Jin. Investigation: Zhongming Li, Qiutao Wang, and Tong Chen. Data curation: Tong Chen. Writing—original draft: Xuedong Fang. Writing—review and editing: Qiutao Wang.

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Correspondence to Xuedong Fang.

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Chen, T., Jin, N., Zhang, Q. et al. Auraptene Mitigates Colitis Induced by Dextran Sulfate Sodium in Mice by Regulating Specific Intestinal Flora and Repairing the Intestinal Barrier. Inflammation (2024). https://doi.org/10.1007/s10753-023-01965-5

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  • DOI: https://doi.org/10.1007/s10753-023-01965-5

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