Abstract
Inflammatory response in the pulmonary endothelium drives the pathogenesis of acute lung injury and sepsis. Sirtuin 6 (SIRT6), a member of class III NAD+-dependent deacetylases belonging to the sirtuin family, regulates senescence, metabolism, and inflammation and extends lifespan in mice and model organisms. However, the role of SIRT6 in pulmonary endothelial inflammation is unknown. Thus, we hypothesized that SIRT6 suppresses inflammatory response in human lung microvascular cells (HLMEC) and ensues monocyte adhesion to endothelial cells. Primary HLMECs were treated with control or SIRT6 adenovirus or SIRT6 agonist, with or without lipopolysaccharide (LPS) treatment. We observed that treatment with LPS did not affect the protein expression of SIRT6 in HLMECs. However, adenovirus-mediated SIRT6 overexpression attenuated LPS-induced VCAM1 gene and protein expression, followed by decreased monocyte adhesion to endothelial cells. Similarly, activation of SIRT6 by a recently reported SIRT6 activator UBCS039, but not the regioisomer negative control compound UBCS060, ameliorated LPS-induced VCAM1 mRNA and protein expression as well as monocyte adhesion. Moreover, luciferase assay revealed that SIRT6 adenovirus decreased the activity of NF-κB, the master regulator of vascular inflammation. Taken together, these results indicate that molecular and pharmacological activation of SIRT6 protects against lung microvascular inflammation via suppressing NF-κB activation, implicating the therapeutic potential of the SIRT6 activators for lung disorders associated with microvascular inflammation.
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The data that support the findings of this study are available from the corresponding author upon reasonable request.
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Funding
This study was partly supported by grants from Guangdong Basic and Applied Basic Research Foundation (no. 2021A1515011001 to JPW), from FISR2019_00374 (MeDyCa) (to AM), and from USA National Institute of Health (HL130167 and HL141171 to ZGJ).
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SWX and ZGJ participated in research design. JPW, JQL, AM, CS, and WZ conducted experiments. JPW, SWX, and ZGJ performed data analysis. JPW and SWX contributed to the writing of the manuscript. All authors have read and approved the final manuscript.
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Wang, J., Luo, J., Rotili, D. et al. SIRT6 Protects Against Lipopolysaccharide-Induced Inflammation in Human Pulmonary Lung Microvascular Endothelial Cells. Inflammation 47, 323–332 (2024). https://doi.org/10.1007/s10753-023-01911-5
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DOI: https://doi.org/10.1007/s10753-023-01911-5