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Tadalafil Alleviates LPS-Induced Inflammation and Oxidative Stress of RWPE-1 Cell by Regulating the Akt/Nrf2 Signaling Pathway

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Abstract

Tadalafil (TAD) is primarily a treatment drug for erectile dysfunction. Studies have shown that TAD has a therapeutic effect on prostatitis, but the specific mechanism has not been reported. LPS induced RWPE-1 cells to form a model of chronic nonbacterial prostatitis (CNP). Cell activity was measured by MTT assay. Apoptosis was detected by TUNEL assay. Western blot was used to detect the expression of apoptosis-related proteins Bcl-2, Bax, Caspase-3, and cleaved caspase3. ELISA was used to detect the expression of inflammatory cytokines TNF-α, IL-6, and IL-8. GSH, catalase (CAT), and malondialdehyde (MDA) kits were used to detect the expression of oxidative stress–related indicators GSH, CAT, and MDA. Western blot was used to detect the expression of proteins related to Akt/Nrf2 signaling pathway. After different concentrations of TAD were given, the survival rate of LPS-induced RWPE-1 cells decreased, apoptosis increased, and inflammation and oxidative stress decreased. This process is accompanied by the activation of the Akt/Nrf2 signaling pathway. The addition of AKT inhibitor (HY-10249A) reversed the inhibitory effect of TAD on LPS-induced inflammatory response and oxidative stress of RWPE-1 cell. TAD alleviated LPS-induced inflammation and oxidative stress of RWPE-1 cell by regulating the Akt/Nrf2 signaling pathway.

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Data Availability

The analyzed data sets generated during the present study are available from the corresponding author on reasonable request.

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Author notes

  1. Xiaoqing Song and Guodong Chen contributed equally to this work.

Authors and Affiliations

  1. Center for Reproductive Medicine, The 904th Hospital of Joint Logistic Support Force, PLA, 101 Xingyuan North Road, Wuxi, 214043, Jiangsu, China

    Xiaoqing Song, Caixia Li, Chunyan Yang & Yun Deng

  2. Center for Reproductive Medicine, The Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi, 214023, Jiangsu, China

    Guodong Chen

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  1. Xiaoqing Song
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  2. Guodong Chen
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  3. Caixia Li
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  4. Chunyan Yang
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  5. Yun Deng
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Correspondence to Yun Deng.

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Song, X., Chen, G., Li, C. et al. Tadalafil Alleviates LPS-Induced Inflammation and Oxidative Stress of RWPE-1 Cell by Regulating the Akt/Nrf2 Signaling Pathway. Inflammation 44, 890–898 (2021). https://doi.org/10.1007/s10753-020-01384-w

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  • DOI: https://doi.org/10.1007/s10753-020-01384-w

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