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Gene Expression in Human Polymorphonuclear Neutrophils (PMNs) Stimulated by Bacillus Calmette–Guérin (BCG)

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Abstract

Neutrophils are the most abundant leukocytes in the blood. Moreover, neutrophils form the first line of host immune defense against bacterial and fungal invasion, and also play an important part in inflammatory and immune system responses. Intravesical bacillus Calmette–Guérin (BCG) has been shown to reduce and delay tumor progression to muscle-invasive disease after transurethral resection of bladder tumors (TRUBTs). Following intravesical BCG, neutrophils gather around tissues infected by BCG in the early stage of inflammatory and immune responses. In our previous study, we reported that BCG induced the formation of neutrophil extracellular traps (NETs), which play an important role in tumor treatment. Therefore, in the present study, we analyzed the gene expression profile of neutrophils stimulated by BCG through high-throughput arrays, which helped us determine the potential roles of neutrophils in BCG immunotherapy. The results showed that the expression of neutrophil genes led to changes in the early stage of BCG stimulation. The changed genes were involved in many functions of neutrophils such as mobility, proliferation, and secretion of cytokines, chemokines, and adhesion molecules. These changes in neutrophil biological functions may play an essential role in BCG induction of inflammatory and immune responses, and in anti-tumor processes.

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Abbreviations

BCG:

bacillus Calmette–Guérin

NMIBC:

non-muscle-invasive bladder cancer

MIBC:

muscle-invasive bladder cancer

TRUBT:

transurethral resection of bladder tumor

LPS:

lipopolysaccharide

PMN:

polymorphonuclear neutrophils

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Acknowledgments

This work was supported by The Second Hospital of Tianjin Medical University (2019ydey12).

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Authors and Affiliations

  1. Department of Urology, Tianjin Institute of Urology, The Second Hospital of Tianjin Medical University, No. 23, PingJiang Road, Hexi District, Tianjin, 300211, People’s Republic of China

    Kangkang Liu, Erlin Sun & Lining Wang

  2. Department of Urology, Tianjin Nankai hospital, Tianjin, 300100, People’s Republic of China

    Xuewu Nian & Chengquan Ma

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Kangkang Liu, Erlin Sun, and Lining Wang contributed to the work equally and should be regarded as co-first authors.

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Liu, K., Sun, E., Wang, L. et al. Gene Expression in Human Polymorphonuclear Neutrophils (PMNs) Stimulated by Bacillus Calmette–Guérin (BCG). Inflammation 43, 2098–2108 (2020). https://doi.org/10.1007/s10753-020-01277-y

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