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Effect of a Stellate Ganglion Block on Acute Lung Injury in Septic Rats

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Abstract

A stellate ganglion block (SGB) is a clinical sympathetic block which can inhibit the body systemic inflammatory response. However, whether and how SGB can attenuate the sepsis-induced acute lung injury remains unclear. Here, we evaluated the effect of SGB on sepsis-induced acute lung injury in rats. Ninety healthy Sprague Dawley (SD) male rats were divided into three groups: the sham operation group (S group), sepsis group (Sep group), and SGB group. The sepsis model rats were produced by cecum ligation and puncture (CLP), and blood samples were taken from the abdominal aorta of the rats at different time points for evaluating the concentration of TNF-α, IL-6, and IL-10 by enzyme-linked immunosorbent assay (ELISA). The rats were sacrificed, and lungs were collected to measure the wet/dry (W/D) lung tissue weight ratio, score the lung tissue pathological damage by microscopic examination, determine the myeloperoxidase (MPO) activity by spectrophotometry, and measure nuclear factor-kappa B (NF-κB) p65 expression by Western blot. The concentration of serum TNF-α, IL-6, and IL-10, lung tissue W/D ratio, pathological injury score, MPO activity, and expression of NF-κB p65 were higher in the Sep group compared with the S group at T1–4. Furthermore, the concentration of serum TNF-α and IL-6, lung tissue W/D ratio, pathological damage score, MPO activity, and expression of NF-κB p65 were reduced and the concentration of IL-10 was increased in the SGB group compared with the Sep group at T1–4. The successful sepsis model rats were induced by CLP, and SGB attenuated the sepsis-induced acute lung injury in rats.

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References

  1. Hennessy, E., C. Adams, F.J. Reen, and F. Is O'Gara. 2016. There potential for repurposing statins as novel antimicrobials? Antimicrobial Agents and Chemotherapy 60 (9): 5111–5121.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  2. Sevransky, J.E., G.S. Martin, C. Shanholtz, et al. 2009. Mortality in sepsis versus non-sepsis induced acute lung injury. Critical Care 13 (5): 1.

    Article  Google Scholar 

  3. Lange, M., Y. Nakano, D.L. Traber, A. Hamahata, A. Esechie, C. Jonkam, K. Bansal, L.D. Traber, and P. Enkhbaatar. 2010. Role of different nitric oxide synthase isoforms in a murine model of acute lung injury and sepsis. Biochemical and Biophysical Research Communications 399 (2): 286–291.

    Article  PubMed  CAS  Google Scholar 

  4. Dulloo, A.G., and J.P. Montani. 2012. Body composition, inflammation and thermogenesis in pathways to obesity and the metabolic syndrome: an overview. Obesity Reviews 13 (S2): 1–5.

    Article  PubMed  Google Scholar 

  5. Hadjiminas, D.J., K.M. McMasters, J.C. Peyton, M.D. Cook, and W.G. Cheadle. 1994. Passive immunization against tumor necrosis factor and interleukin-1 fails to reduce lung neutrophil sequestration in chronic sepsis. Shock 2 (5): 376–380.

    Article  PubMed  CAS  Google Scholar 

  6. Bai, J., L. Tang, J. Lomasneira, et al. 2015. TAT-SNAP-23 treatment inhibits the priming of neutrophil functions contributing to shock and/or sepsis-induced extra-pulmonary acute lung injury. Innate Immunity 21 (1): 42–54.

    Article  PubMed  CAS  Google Scholar 

  7. Dan, H., X. Yang, Y. Xiang, et al. 2015. Inhibition of Toll-like receptor 9 attenuates sepsis-induced mortality through suppressing excessive inflammatory response. Cellular Immunology 295 (2): 92–98.

    Article  CAS  Google Scholar 

  8. Toklu, H.Z., T.T. Akbay, A. Velioglu-Ogunc, et al. 2008. Silymarin, the antioxidant component of Silybum marianum, prevents sepsis-induced acute lung and brain injury. Journal of Surgical Research 145 (2): 214–222.

    Article  PubMed  CAS  Google Scholar 

  9. Hynninen, M., V. Pettilä, O. Takkunen, R. Orko, S.E. Jansson, P. Kuusela, R. Renkonen, and M. Valtonen. 2003. Predictive value of monocyte histocompatibility leukocyte antigen-DR expression and plasma interleukin-4 and -10 levels in critically ill patients with sepsis. Shock 20 (1): 1–4.

    Article  PubMed  CAS  Google Scholar 

  10. Chen, X., Y. Wang, H. Luo, et al. 2013. Ulinastatin reduces urinary sepsis-related inflammation by upregulating IL-10 and downregulating TNF-α levels. Molecular Medicine Reports 8 (1): 29–34.

    Article  PubMed  CAS  Google Scholar 

  11. Liu, G.-D., and C.-J. He. 2014. Stellate ganglion block promotes recovery of Bell’s palsy in patients with diabetes mellitus. Acta Oto-Laryngologica 134 (6): 652–655.

    Article  PubMed  CAS  Google Scholar 

  12. Noma, N., H. Kamo, Y. Nakaya, K. Dezawa, A. Young, J. Khan, and Y. Imamura. 2013. Stellate ganglion block as an early intervention in sympathetically maintained headache and orofacial pain caused by temporal arteritis. Pain Medicine 14 (3): 392–397.

    Article  PubMed  Google Scholar 

  13. Liu, M.H., J. Tian, Y.P. Su, T. Wang, Q. Xiang, and L. Wen. 2013. Cervical sympathetic block regulates early systemic inflammatory response in severe trauma patients. Medical Science Monitor International Medical Journal of Experimental & Clinical Research 19: 194–201.

    Article  CAS  Google Scholar 

  14. Liu, M.-H., J. Tian, Y.-P. Su, T. Wang, Q. Xiang, and L. Wen. 2013. Cervical sympathetic block regulates early systemic inflammatory response in severe trauma patients. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research 19: 194.

    Article  CAS  Google Scholar 

  15. Cui, Y., L. Wang, Z. Tian, Z. Lin, and D. Chen. 2013. The effect of rhubarb pre-treatment on intestinal microcirculation in septic rats. American Journal of Chinese Medicine 42 (5): 1215–1227.

    Article  Google Scholar 

  16. Ikeda, T., H. Hirakawa, T. Kemuriyama, Y. Nishida, and T. Kazama. 2009. Effect of cervical sympathetic trunk transection on renal sympathetic nerve activity in rats. Physiological Research 58 (1): 77–82.

    PubMed  CAS  Google Scholar 

  17. Liu, M.H., J. Tian, Y.P. Su, T. Wang, Q. Xiang, and L. Wen. 2012. Cervical sympathetic block regulates early systemic inflammatory response in severe trauma patients. Medical Science Monitor International Medical Journal of Experimental & Clinical Research 19: 194–201.

    Google Scholar 

  18. Ghosh, S., and M.S. Hayden. 2008. New regulators of NF-κB in inflammation. Nature Reviews Immunology 8 (11): 837–848.

    Article  PubMed  CAS  Google Scholar 

  19. Li, Z., S. Jin, C. Wang, R. Jiang, and J. Wan. 2010. Histone deacetylase inhibitors attenuate acute lung injury during cecal ligation and puncture-induced polymicrobial sepsis. World Journal of Surgery 34 (7): 1676–1683.

    Article  Google Scholar 

  20. Wang, Y.-H., T.-H. Li, B.-Q. Wu, H. Liu, Y.-F. Shi, and D.-Y. Feng. 2015. Protective effects of caffeoylxanthiazonoside isolated from fruits of Xanthium strumarium on sepsis mice. Pharmaceutical Biology 53 (9): 1367–1371.

    Article  PubMed  CAS  Google Scholar 

  21. Iwama, H. 1997. Can superior cervical ganglionectomy be an experimental model of stellate ganglion block? Masui the Japanese Journal of Anesthesiology 46 (4): 565–567.

    PubMed  CAS  Google Scholar 

  22. Deleon, C., K. Shattuck, and S.K. Jain. 2015. Biomarkers of neonatal sepsis. NeoReviews 16 (5): e297-e308.

    Article  Google Scholar 

  23. Kolyva, A., V. Zolota, D. Mpatsoulis, et al. 2014. The role of obesity in the immune response during sepsis. Nutrition & Diabetes 4 (9): e137.

    Article  CAS  Google Scholar 

  24. Schuerholz, T., S. Doemming, M. Hornef, et al. 2013. The anti-inflammatory effect of the synthetic antimicrobial peptide 19-2.5 in a murine sepsis model: a prospective randomized study. Critical Care 17 (1): 1.

    Article  Google Scholar 

  25. Valdés-Ferrer, S.I., J. Papoin, M.E. Dancho, et al. 2015. HMGB1 mediates anemia of inflammation in murine sepsis survivors. Molecular Medicine 21 (1): 951.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  26. Gokmen, Z., S. Ozkiraz, S. Kulaksizoglu, et al. 2013. Resistin—a novel feature in the diagnosis of sepsis in premature neonates. American Journal of Perinatology 30 (06): 513–518.

    Article  PubMed  Google Scholar 

  27. Botez, G., G. Piraino, P.W. Hake, J.R. Ledford, M. O’Connor, J.A. Cook, and B. Zingarelli. 2015. Age-dependent therapeutic effects of liver X receptor-α activation in murine polymicrobial sepsis. Innate Immunity 21 (6): 609–618.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  28. Zhu, T., D.-X. Wang, W. Zhang, X.Q. Liao, X. Guan, H. Bo, J.Y. Sun, N.W. Huang, J. He, Y.K. Zhang, J. Tong, and C.Y. Li. 2013. Andrographolide protects against LPS-induced acute lung injury by inactivation of NF-κB. PLoS One 8 (2): e56407.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  29. Su, N., K. Miao, X. Zhou, et al. 2014. The involvement of follistatin-like protein 1 in osteoarthritis by elevating NF-κB-mediated inflammatory cytokines and enhancing fibroblast like synoviocyte proliferation. Arthritis Research & Therapy 17 (1): 1–10.

    Google Scholar 

  30. Kothari, N., J. Bogra, H. Abbas, M. Kohli, A. Malik, D. Kothari, S. Srivastava, and P.K. Singh. 2013. Tumor necrosis factor gene polymorphism results in high TNF level in sepsis and septic shock. Cytokine 61 (2): 676–681.

    Article  PubMed  CAS  Google Scholar 

  31. Khowailed, A., S.M. Younan, H. Ashour, A.E. Kamel, and N. Sharawy. 2015. Effects of ghrelin on sepsis-induced acute kidney injury: one step forward. Clinical and Experimental Nephrology 19 (3): 419–426.

    Article  PubMed  CAS  Google Scholar 

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Funding

This work was supported by grants of National Natural Science Foundation of China (81560193) (GX), Science and Technology Research Project of Education Department of Jiangxi Province (150258) (YC), and the Young Fund of Science and Technology Plan of Second Affiliated Hospital of Nanchang University (2016YNQN12008) (YC).

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    Authors and Affiliations

    1. Department of Anesthesiology, The Second Affiliated Hospital of Nanchang University, NO.1 Mingde Road, Nanchang, 330006, China

      Yong Chen, Lian Guo, Haili Lang, Xiaolan Hu, Sun Jing, Mengsi Luo, Guohai Xu & Zhidong Zhou

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    1. Yong Chen
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    2. Lian Guo
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    3. Haili Lang
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    4. Xiaolan Hu
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    8. Zhidong Zhou
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    Correspondence to Zhidong Zhou.

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    All experiments were approved by the Ethics Committee of Second Affiliated Hospital of Nanchang University in Nanchang, China.

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    Yong Chen and Lian Guo contributed equally to this work.

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    Chen, Y., Guo, L., Lang, H. et al. Effect of a Stellate Ganglion Block on Acute Lung Injury in Septic Rats. Inflammation 41, 1601–1609 (2018). https://doi.org/10.1007/s10753-018-0803-x

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