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The Elevated Serum S100A8/A9 During Acute Myocardial Infarction Is Not of Cardiac Myocyte Origin

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Abstract

Overproduction of circulating S100A8/A9 occurs in patients following acute myocardial infarction (AMI). It remains unclear whether ischemia insult per se induces S100A8 and S100A9 expression in cardiac myocytes or even whether the cardiac myocytes participate as a source of these proteins. In this study, western blot analysis and quantitative real-time reverse transcription polymerase chain reaction were used to test samples obtained from isolated spontaneously hypertensive rat hearts and Wistar-Kyoto rat hearts subjected to global normothermic ischemia and from neonatal Wistar rat cardiac myocytes undergoing hypoxia. Ischemia did not increase the expression of S100A8 and S100A9 proteins and mRNA in the myocardium either from the spontaneously hypertensive rat hearts or the Wistar-Kyoto rat hearts. In addition, the levels of S100A8 and S100A9 proteins were unchanged in the neonatal rat cardiac myocytes undergoing hypoxia. However, both ischemia and hypoxia activated NF-kappaB in ischemic myocardium and in hypoxic cardiac cells in a time-dependent manner. The results suggest that the increased serum S100A8/A9 concentrations following AMI were not of cardiac myocyte origin.

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Abbreviations

ACS:

Acute coronary syndrome

AMI:

Acute myocardial infarction

SHR:

Spontaneously hypertensive rats

WKY:

Wistar-Kyoto rats

PBS:

Phosphate-buffered saline

LVEDP:

Left ventricular end-diastolic pressure

LVDP:

Left ventricular developed pressure

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

RT-qPCR:

Quantitative real-time reverse transcription polymerase chain reaction

LPS:

Lipopolysaccharide

PMN:

Polymorphonuclear leukocytes

IL-1β:

Interleukin-1 beta

TNF-α:

Tumor necrosis factor alpha

IL-1α:

Interleukin-1 alpha

TGF-β:

Transforming growth factor beta

RAGE:

Receptor for advanced glycosylation end products

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Acknowledgments

This work was supported by the National Natural Sciences Foundation of China (project numbers 30470715, 30870939) and the Research Fund for the Doctoral Program of Higher Education of China (no. 20040335118).

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The authors have no conflicting financial interests.

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Authors and Affiliations

  1. Institute of Cardiology, the First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qing Chun Road, Hangzhou, Zhejiang, 310003, People’s Republic of China

    Chang-Qing Du, Lin Yang, Jie Han, Jian Yang, Xue-Yan Yao, Xiao-Sheng Hu & Shen-Jiang Hu

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  1. Chang-Qing Du
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  2. Lin Yang
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  3. Jie Han
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Correspondence to Shen-Jiang Hu.

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Chang-Qing Du and Lin Yang contributed equally to this work.

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Du, CQ., Yang, L., Han, J. et al. The Elevated Serum S100A8/A9 During Acute Myocardial Infarction Is Not of Cardiac Myocyte Origin. Inflammation 35, 787–796 (2012). https://doi.org/10.1007/s10753-011-9375-8

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