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The effect of hyperbaric oxygen treatment on aspiration pneumonia

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Abstract

We have studied whether hyperbaric oxygen (HBO) prevents different pulmonary aspiration materials-induced lung injury in rats. The experiments were designed in 60 Sprague-Dawley rats, ranging in weight from 250 to 300 g, randomly allotted into one of six groups (n = 10): saline control, Biosorb Energy Plus (BIO), hydrochloric acid (HCl), saline + HBO treated, BIO + HBO treated, and HCl + HBO treated. Saline, BIO, HCl were injected into the lungs in a volume of 2 ml/kg. A total of seven HBO sessions were performed at 2,4 atm 100% oxygen for 90 min at 6-h intervals. Seven days later, rats were sacrificed, and both lungs in all groups were examined biochemically and histopathologically. Our findings show that HBO inhibits the inflammatory response reducing significantly (P < 0.05) peribronchial inflammatory cell infiltration, alveolar septal infiltration, alveolar edema, alveolar exudate, alveolar histiocytes, interstitial fibrosis, granuloma, and necrosis formation in different pulmonary aspiration models. Pulmonar aspiration significantly increased the tissue HP content, malondialdehyde (MDA) levels and decreased (P < 0.05) the antioxidant enzyme (SOD, GSH-Px) activities. HBO treatment significantly (P < 0.05) decreased the elevated tissue HP content, and MDA levels and prevented inhibition of SOD, and GSH-Px (P < 0.05) enzymes in the tissues. Furthermore, there is a significant reduction in the activity of inducible nitric oxide synthase, TUNEL and arise in the expression of surfactant protein D in lung tissue of different pulmonary aspiration models with HBO therapy. It was concluded that HBO treatment might be beneficial in lung injury, therefore, shows potential for clinical use.

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Acknowledgments

This study was supported as Project TUBAB 2010-179 by Trakya University Research Center, Edirne, Turkey.

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Authors and Affiliations

  1. Department of Anesthesiology and Reanimation, Faculty of Medicine, Trakya University, 22030, Edirne, Turkey

    Sevtap Hekimoglu Sahin & Alkin Colak

  2. Department of Histology and Embryology, Faculty of Medicine, Trakya University, 22030, Edirne, Turkey

    Mehmet Kanter & Mustafa Erboga

  3. Department of Pediatric Surgery, Faculty of Medicine, Trakya University, 22030, Edirne, Turkey

    Suleyman Ayvaz, Burhan Aksu & Umit Nusret Basaran

  4. Department of Pediatrics, Faculty of Medicine, 19 Mayis University, 22030, Samsun, Turkey

    Ahmet Guzel

  5. Department of Biochemistry, Private Central Hospital, Kozyatagi, Istanbul, Turkey

    Ali Ozcan

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  1. Sevtap Hekimoglu Sahin
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  2. Mehmet Kanter
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  3. Suleyman Ayvaz
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  4. Alkin Colak
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Correspondence to Mehmet Kanter.

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Sahin, S.H., Kanter, M., Ayvaz, S. et al. The effect of hyperbaric oxygen treatment on aspiration pneumonia. J Mol Hist 42, 301–310 (2011). https://doi.org/10.1007/s10735-011-9334-6

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  • DOI: https://doi.org/10.1007/s10735-011-9334-6

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