Abstract
Jasmonic acid (JA) and salicylic acid (SA) are plant hormones involved in plant growth and development. Recent studies demonstrated that presence of a complex interplay between JA and SA signaling pathways to response to pathogenesis attack and biotic stresses. To our best knowledge, no method has existed for simultaneous analyses of JA, SA, and their related compounds. Especially, the glucosides are thought to be the storages or the inactivated compounds, but their contribution should be considered for elucidating the amount of the aglycons. It is also valuable for measuring the endogenous amount of phenylalanine, cinnamic acid, and benzoic acid that are the biosynthetic intermediates of SA due to the existence of isochorismate pathway to synthesize SA. We established this method using deuterium labeled compounds as internal standards. This is the first report of simultaneous analysis of endogenous JA, SA, and their related compounds. Measuring the endogenous JA, SA, and their related compounds that had been accumulated in tobacco plants proved the practicality of the newly developed method. It was demonstrated that accumulation of JA, SA and their related compounds were induced in both case of TMV infection and abiotic stresses.
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Abbreviations
- JA:
-
Jasmonic acid
- MRM:
-
Multiple reaction monitoring
- MS:
-
Mass spectrometry
- MS/MS:
-
Tandem mass spectrometry
- SA:
-
Salicylic acid
- SAG:
-
Salicylic acid glucoside
- TA:
-
Tuberonic acid
- TAG:
-
Tuberonic acid glucoside
- TMV:
-
Tobacco mosaic virus
- UPLC:
-
Ultra performance liquid chromatography
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Acknowledgments
The authors are grateful to Mr. Kenji Watanabe and Dr. Eri Fukushi for measuring the NMR and mass spectra.
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Matsuura, H., Aoi, A., Satou, C. et al. Simultaneous UPLC MS/MS analysis of endogenous jasmonic acid, salicylic acid, and their related compounds. Plant Growth Regul 57, 293–301 (2009). https://doi.org/10.1007/s10725-008-9347-7
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DOI: https://doi.org/10.1007/s10725-008-9347-7