Abstract
Mutation is prerequisite for creating new variation in crop plants. This variation forms the basis for any crop improvement program. In this study, a population of 3774 mungbean M1 mutant plants were generated using 0.7% of ethyl methane sulfonate (EMS). For TILLING by sequencing, 768 M2 families were sequenced for three different candidate genes responsible for altering plant architecture in mungbean. In total, five exon residing mutations were identified, among them three mutations each in GIGANTEA (GI), RAMOSUS (RMS) and TERMINAL FLOWER1 (TFL1) were found to be deleterious and altering the protein function based on SIFT analysis. Through morphological characterization, we confirmed the potential role of each mutation in altering mungbean plant architecture to develop an ideal plant type to increase the yield potential. Additionally, mutants with desirable traits such as increase in number of branches, and terminal and late flowering were selected from this study and advanced to further generation, as well as we are planning to pyramiding all three mutation in single genetic background that could contribute potentially towards developing high yielding mungbean genotypes with ideal plant type.
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Acknowledgements
This work was supported by Department of Biotechnology, Government of India under TILLING by sequencing of morphogenesis and flowering control genes to augment ideotype breeding in Mungbean.
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AV and BRR performed the experiments, data analysis and drafted the manuscript, SG, and RB helped in data analysis and execution of this study, SGR and GK conceptualized this study and provided lab facility and guidance for drafting the manuscript. All authors read and approved the final manuscript.
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Varadaraju, A., Ramadoss, B.R., Gurunathan, S. et al. TILLING by sequencing (TbyS) reveals mutations in flowering control genes that are associated with altered plant architecture in Mungbean (Vigna radiata (L.) R. Wilczek). Genet Resour Crop Evol 68, 849–864 (2021). https://doi.org/10.1007/s10722-020-01028-w
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DOI: https://doi.org/10.1007/s10722-020-01028-w