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Upregulation of Fucosyltransferase 3, 8 and protein O-Fucosyltransferase 1, 2 genes in esophageal cancer stem-like cells (CSLCs)

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Abstract

Recently, studies have shown that Fucosylation plays an important role in the invasion and metastatic process of CSLCs. Understanding the expression pattern of fucosyltransferase (FUT) genes may help to suggest better-targeted therapy strategies for esophageal squamous cell carcinoma (ESCC). The study aimed to address the expression pattern of FUT gene variants in esophageal CSLCs and parental adherent cells. Sphere formation method was used to enrich CSLCs. Expression of FUT genes was examined in tumor sphere and parental adherent cells using the RT-PCR method and then relative expression of detected variants was performed by the Real-Time PCR method in both groups. The detected FUTs, also, were assessed in fresh ESCC tumors and the matched healthy controls. Analysis of The cell surface carbohydrate Lewis x (LeX, CD15) was performed by flow cytometry. Molecular analysis showed that the expression of FUT 3, 8 and POFUT1, 2 genes in tumorsphere were significantly higher than parental adherent cells. Analysis of fresh ESCC tumor tissues and the matched healthy controls showed that FUT8 and POFUT1, 2 genes in contrast to FUT 3 have higher expression in tumor tissues than controls. Flow cytometric analyses revealed that tumorsphere and their parent cells do not differ significantly in Lewis x surface marker. The present study showed that FUT 3, 8 and POFUT1, 2 genes upregulated in esophageal CSLCs in comparison to adherent cells. Understanding the expression pattern of FUT gene variants may help to suggest better-targeted therapy strategies for ESCC.

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Acknowledgments

We thank Dr. Masoud khoshnia for providing ESCC and normal samples, and Dr. seyed Mehdi Jafari for editing the manuscript. Special thanks to the patients with esophageal cancer who participated in this study. This work was supported by the Golestan University of Medical Sciences (research grant number: 970308034).

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Authors and Affiliations

  1. Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran

    Zahra Sadeghzadeh, Marie Saghaeian Jazi & Jahanbakhsh Asadi

  2. Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran

    Ayyoob Khosravi, Marie Saghaeian Jazi & Jahanbakhsh Asadi

  3. Department of Molecular Medicine, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran

    Ayyoob Khosravi

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  1. Zahra Sadeghzadeh
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Sadeghzadeh, Z., Khosravi, A., Jazi, M.S. et al. Upregulation of Fucosyltransferase 3, 8 and protein O-Fucosyltransferase 1, 2 genes in esophageal cancer stem-like cells (CSLCs). Glycoconj J 37, 319–327 (2020). https://doi.org/10.1007/s10719-020-09917-z

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