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Polysaccharide structural variability in mycobacteria: identification and characterization of phosphorylated mannan and arabinomannan

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Abstract

Arabinomannan (AMannan) and mannan (Mannan) are major polysaccharides antigens of the mycobacterial capsule. They are highly related to the lipoarabinomannan (LAM) and lipomannan (LM) lipoglycans of the cell wall, known to participate to the immunopathogenesis of mycobacterial infections. Here we present the identification of two related polysaccharides from Mycobacterium kansasii that co-purified with AMannan and Mannan. Structural analysis using GC, MALDI-MS and NMR clearly established these molecules as non-acylated phosphorylated AMannan and Mannan designated P-AMannan and P-Mannan, respectively. These glycoconjugates represent a new source of polysaccharide structural variability in mycobacteria and constitute unique tools for structure-activity relationship studies in order to investigate the role of fatty acids in the biological functions of LAM and LM. The potential participation of these polysaccharides in influencing the outcome of the infection is also discussed.

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Abbreviations

Ara:

arabinose

ConA:

concanavalin A

GC:

gas-chromatography

HPAEC:

high pH anion exchange chromatography

Ins:

inositol

MALDI-TOF:

matrix assisted lazer desorption ionisation coupled to time of flight analyser

Man:

mannose

Man-ol:

mannitol

Me-Glc:

methyl glucoside

NMR:

nuclear magnetic resonance

TFA:

trifluoroacetic acid

TLC:

thin layer chromatography

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Acknowledgments

This study was funded by a grant from the french National Research Agency (ANR-05-MIIM-025). LK is supported by a grant from the Centre National de la Recherche Scientifique (CNRS) (Action Thématique Incitative sur Programme “Microbiologie Fondamentale”).

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Authors and Affiliations

  1. Unité de Glycobiologie Structurale et Fonctionnelle, UMR 8576 CNRS, Université des Sciences et Technologies de Lille, 59655, Villeneuve d’Ascq cedex, France

    Emmanuel Maes, Bernadette Coddeville & Yann Guérardel

  2. Laboratoire de Dynamique des Interactions Membranaires Normales et Pathologiques, UMR 5235 CNRS, Université des Sciences et Techniques du Languedoc (Montpellier II), 34095, Montpellier cedex 5, France

    Laurent Kremer

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  1. Emmanuel Maes
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  2. Bernadette Coddeville
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  4. Yann Guérardel
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Correspondence to Yann Guérardel.

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Maes, E., Coddeville, B., Kremer, L. et al. Polysaccharide structural variability in mycobacteria: identification and characterization of phosphorylated mannan and arabinomannan. Glycoconj J 24, 439–448 (2007). https://doi.org/10.1007/s10719-007-9036-1

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  • DOI: https://doi.org/10.1007/s10719-007-9036-1

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