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Population structure and reproductive mode of Dothistroma septosporum in the Brittany peninsula of France

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Abstract

Dothistroma needle blight (DNB) is regarded as one of the most significant foliar diseases of pine and has caused significant damage to pine plantations in France since the late 1990s. The genetic diversity, reproductive strategy and population structure of D. septosporum, one of the causal agents of DNB, was investigated at six sites across Brittany, France, using species-specific mating type primers and microsatellite markers. Multiplex PCR assays were developed to effectively and efficiently screen 11 of the available microsatellite markers on the isolates obtained. From 282 isolates analysed, 81 unique multilocus haplotypes were identified, indicating a diverse (haplotypic diversity ranged from 0.403 to 0.962) yet highly clonal structure of the pathogen at these sites. Although all sites contained both mating types, random mating was rejected by the index of association and parsimony tree length permutation tests on both clone-corrected and non-clone-corrected datasets but supported by mating type ratios in the clone-corrected datasets. Four population clusters were identified using STRUCTURE. Neither locality nor host species played a significant role in the population structure in Brittany. These findings, along with the observation of identical haplotypes at different sites and on different hosts, suggest movement of the pathogen within the peninsula. Overall, the pathogen’s population structure was found to be influenced by sexual recombination yet within sites clonal reproduction predominated.

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Acknowledgments

The authors thank Benoît Marçais for supplying information about survey sites, and Helen Hipperson for help with microsatellite analysis, as well as advice and support from Simon Archer (Imperial College, London). The authors are grateful for the helpful comments of Joan Webber and Kath Tubby which helped improve the manuscript. This study was financially supported by the Forestry Commission, UK and Imperial College London. The authors would like to acknowledge networking support by the COST Action FP1102, DIAROD and the Department of Science and Technology (DST) and ESTAP, South Africa.

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Authors and Affiliations

  1. Forest Research, Alice Holt Lodge, Farnham, Surrey, GU10 4LH, UK

    M. S. Mullett & A. V. Brown

  2. Imperial College London, Silwood Park Campus, Buckhurst Road, Ascot, Berks, SL5 7PY, UK

    M. S. Mullett

  3. Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, 0002, South Africa

    I. Barnes

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  1. M. S. Mullett
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Correspondence to M. S. Mullett.

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Mullett, M.S., Brown, A.V. & Barnes, I. Population structure and reproductive mode of Dothistroma septosporum in the Brittany peninsula of France. Eur J Plant Pathol 143, 261–275 (2015). https://doi.org/10.1007/s10658-015-0678-8

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