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Vegetative compatibility groups in Verticillium dahliae isolates from olive in western Turkey

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Abstract

Verticillium wilt, caused by Verticillium dahliae, is the most serious disease in olive cultivation areas in western Turkey. Two hundred and eight isolates of V. dahliae from olive (Olea europea var. sativa) trees were taken for vegetative compatibility analysis using nitrate non-utilizing (nit) mutants. One isolate did not produce a nit mutant. Nit mutants of 207 isolates were tested against tester strains of internationally known vegetative compatibility groups (VCGs) 1A, 2A, 2B, 3, 4A and 4B, and also paired in many combinations among themselves. One hundred and eighty nine of the isolates (90.9%) were strongly compatible with T9, the tester strain of VCG1A, and thus were assigned to VCG1A. Eight isolates were assigned to VCG2A and four isolates to VCG4B. One isolate was heterokaryon self-incompatible (HSI) and five isolates could not be grouped to any of the VCGs tested. Pathogenicity assays were conducted on a susceptible olive cultivar (O. europea cv. Manzanilla) and a susceptible local cotton cultivar (Gossypium hirsutum cv. Çukurova 1518). Both cotton and olive inoculated with all VCG1A isolates showed defoliating symptoms in greenhouse tests. This is the first report on VCGs in V. dahliae from olive trees in Turkey which demonstrates that VCG1A of the cotton-defoliating type is the most commonly detected form from olive plants in the western part of Turkey.

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Acknowledgements

This research was supported by The Scientific and Technical Research Council of Turkey (TUBITAK). We thank Dr. Nadia Korolev for providing the international reference isolates of the V. dahliae VCGs, Dr. F. Evrendilek and Dr. A. E. Yıldırım for critical reading of manuscript. We also thank Sinem Aldemir and Sezgin Kadıoğlu for their technical assistance.

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Authors and Affiliations

  1. Department of Plant Protection, Faculty of Agriculture, Mustafa Kemal University, 31034, Antakya, Hatay, Turkey

    Sibel Dervis, Soner Soylu, Fatih M. Tok, Sener Kurt & E. Mine Soylu

  2. Olive Research Institute, Turkish Ministry of Agriculture and Rural Affairs, 35100, Bornova, İzmir, Turkey

    Latife Erten

  3. Department of Plant Protection, Faculty of Agriculture, Ege (Aegean) University, 35100, Bornova, İzmir, Turkey

    Mehmet Yıldız

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  1. Sibel Dervis
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  2. Latife Erten
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  3. Soner Soylu
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  7. E. Mine Soylu
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Correspondence to Sibel Dervis.

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Dervis, S., Erten, L., Soylu, S. et al. Vegetative compatibility groups in Verticillium dahliae isolates from olive in western Turkey. Eur J Plant Pathol 119, 437–447 (2007). https://doi.org/10.1007/s10658-007-9183-z

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  • DOI: https://doi.org/10.1007/s10658-007-9183-z

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