Summary
Neuroblastoma (NB) is a common tumor in children, usually in the retroperitoneum. After various treatments, low- and intermediate-risk patients have achieved good results, but the prognosis of high-risk patients is still very poor. Therefore, it is necessary to find new effective targets for the treatment of high-risk patients. In this study, comprehensive bioinformatics analysis was used to identify the differentially expressed genes (DEG and DEM) between high-risk patients and non-high-risk patients, and it was identified that ADRB2 may affect the survival status of high-risk patients due to miR -30a-5p regulation. The GSE49710, GSE73517, and GSE121513 datasets were downloaded from the Gene Expression Synthesis (GEO) database, and DEG and DEM were selected. Then, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were applied to the selected DEGs. The STRING database and Cytoscape software were used to construct protein-protein interaction (PPI) networks and perform modular analysis of the DEGs. The TARGET data set containing information on overall survival days were used for the prognostic analysis of central genes. We identified a total of 255 DEGs from GSE49710 and GSE73517, and 193 DEMs from GSE121513. We identified the 5 most important central genes from the PPI network, performed a prognostic analysis in the target data set, and verified their expression using RT-qPCR to select the most important ADRB2 gene to predict miRNA. Integrating the differential miRNA predicted by miRDB and miRSystem and GSE121513 between the targeted miRNA and the prognosis, miR-30a-5p was finally identified as the targeted miRNA of ADRB2.
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Data availability
The datasets analyzed during the current study are available in the Gene Expression Omnibus (http://www.ncbi.nlm.nih.gov/geo/) and Therapeutically Applicable Research To Generate Effective Treatments (https://ocg.cancer.gov/programs/target) repositories.
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This work was supported by the Key Project of “Research on Prevention and Control of Major Chronic Non-Communicable Diseases”, Ministry of Science and Technology of the People’s Republic of China (2018YFC1313004), Chongqing Science and Technology Commission (cstc2019jscx-msxmX0220); General Clinical Medical Research Program of Children’s Hospital of Chongqing Medical University (YBXM-2019-003).
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Shao performed the research, analyzed the data, and wrote the manuscript, Liu collected the clinical samples, and Wang* contributed to carrying out additional analyses, discussed the results, provided scientific advice, and revised the manuscript. All authors read and approved the final manuscript.
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Shao, FL., Liu, Qq. & Wang, S. Identify potential miRNA-mRNA regulatory networks contributing to high-risk neuroblastoma. Invest New Drugs 39, 901–913 (2021). https://doi.org/10.1007/s10637-021-01064-y
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DOI: https://doi.org/10.1007/s10637-021-01064-y