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RETRACTED ARTICLE: BBOX1-AS1 Accelerates Gastric Cancer Proliferation by Sponging miR-3940-3p to Upregulate BIRC5 Expression

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This article was retracted on 03 November 2022

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Abstract

Background

Gastric cancer (GC) is one type of the most general malignancies in the globe. Research increasingly suggests long non-coding RNAs (lncRNAs) exert crucial roles in GC. However, the function of BBOX1-AS1 in GC has not been reported yet, it needs more explorations.

Aims

The aim of the study is to figure out the role and related regulation mechanism of BBOX1-AS1 in GC.

Methods

RT-qPCR assay was applied to detect genes expression. The role of BBOX1-AS1 in GC was investigated by cell counting kit-8, colony formation, tunel detection, and western blot assays. The binding ability between miR-3940-3p and BBOX1-AS1 (or BIRC5) by RIP, RNA pull-down and luciferase reporter assays.

Results

The expression of BBOX1-AS1 presented significantly upregulation in GC tissues and cells. Moreover, upregulation of BBOX1-AS1 promoted GC cell proliferation, and inhibited GC cell apoptosis. However, downregulation of BBOX1-AS1 led to opposite results. Furtherly, we discovered that BBOX1-AS1 bound with miR-3940-3p and also negatively regulated miR-3940-3p. Besides, it proved that miR-3940-3p interplayed with BIRC5 and negatively regulated BIRC5. Through rescue experiments, we proved that BIRC5 reversed miR-3940-3p-mediated cell proliferation or apoptosis in BBOX1-AS1-dysregulated GC cells.

Conclusions

BBOX1-AS1 accelerates GC proliferation by sponging miR-3940-3p to upregulate BIRC5 expression, which may guide a new direction into the therapeutic strategies of GC.

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Acknowledgments

We appreciate the technical supports of Tengzhou Central People’s Hospital.

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Authors and Affiliations

  1. Department of Gastroenterology, Tengzhou Central People’s Hospital, No. 181, Xingtan Road, Tengzhou, 277500, Shandong, China

    Yan Yang, Bing Li, Renzhen Guan, ChangYong Huang & XiuCheng Yang

  2. Department of Pathology, Zaozhuang Mining Group Central Hospital, Zaozhuang, 277000, Shandong, China

    Qiong Yu

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  1. Yan Yang
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Correspondence to XiuCheng Yang.

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Yan Yang and Qiong Yu—Co-first authors.

This article has been retracted. Please see the retraction notice for more detail:https://doi.org/10.1007/s10620-022-07738-8

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Yang, Y., Yu, Q., Li, B. et al. RETRACTED ARTICLE: BBOX1-AS1 Accelerates Gastric Cancer Proliferation by Sponging miR-3940-3p to Upregulate BIRC5 Expression. Dig Dis Sci 66, 1054–1062 (2021). https://doi.org/10.1007/s10620-020-06308-0

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  • DOI: https://doi.org/10.1007/s10620-020-06308-0

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