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Enhanced Expression of CXCL13 in Human Helicobacter pylori-Associated Gastritis

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Abstract

Background and Aims

Chemokine CXC ligand 13 (CXCL13) and CXC receptor type 5 (CXCR5) are constitutively expressed in tertiary lymphoid follicles where the CXCL13/CXCR5 system regulates B lymphocytes homing. In this study, we sought to examine CXCL13 expression in the H. pylori-infected and -uninfected gastric mucosa and to elucidate the implication in the pathogenesis of HAG in humans.

Methods

Using endoscopic biopsies taken from the gastric antrum of 29 subjects infected with Helicobacter pylori and 22 uninfected subjects, mucosal CXCL13 mRNA and protein levels were measured by real-time polymerase chain reaction and enzyme-linked immunosorbent assay, respectively.

Results

The CXCL13 expression levels were significantly more elevated in H. pylori-positive patients than uninfected ones. The CXCL13 expression levels correlated with the degree of chronic gastritis and bacterial colonization. Immunohistochemistry and in vitro infection assay showed that CXCL13 was not produced by the gastric epithelium, but the α-smooth muscle antigen expressing mesenchymal cells were the possible source of CXCL13 within H. pylori-infected gastric mucosa. CXCR5 immunostaining was seen in the CD20-positive lymphoid aggregates.

Conclusions

The enhanced induction of CXCL13 may be involved in the pathogenesis of H. pylori-associated gastritis.

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Conflict of interests

All authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. Second Department of Internal Medicine, Nagasaki University Hospital, Sakamoto 1-7-1, Nagasaki, 852-8102, Japan

    Yujiro Nakashima & Shigeru Kohno

  2. Department of Gastroenterology and Hepatology, Nagasaki University Hospital, Sakamoto 1-7-1, Nagasaki, 852-8102, Japan

    Hajime Isomoto, Kayoko Matsushima, Akira Yoshida, Tatsuki Ichikawa, Fuminao Takeshima & Kazuhiko Nakao

  3. Department of Pathology, Nagasaki University Hospital, Sakamoto 1-7-1, Nagasaki, 852-8102, Japan

    Toshiyuki Nakayama & Tomayoshi Hayashi

  4. Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Sakamoto 1-12-4, Nagasaki, 852-8102, Japan

    Masaaki Nakayama, Junzo Hisatsune & Toshiya Hirayama

Authors
  1. Yujiro Nakashima
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  2. Hajime Isomoto
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  3. Kayoko Matsushima
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  4. Akira Yoshida
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  5. Toshiyuki Nakayama
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  6. Masaaki Nakayama
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  7. Junzo Hisatsune
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  8. Tatsuki Ichikawa
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  9. Fuminao Takeshima
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  10. Tomayoshi Hayashi
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  11. Kazuhiko Nakao
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  12. Toshiya Hirayama
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  13. Shigeru Kohno
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Corresponding author

Correspondence to Hajime Isomoto.

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Nakashima, Y., Isomoto, H., Matsushima, K. et al. Enhanced Expression of CXCL13 in Human Helicobacter pylori-Associated Gastritis. Dig Dis Sci 56, 2887–2894 (2011). https://doi.org/10.1007/s10620-011-1717-8

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  • DOI: https://doi.org/10.1007/s10620-011-1717-8

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