A novel alkaline-soluble polysaccharide (PVPS-3), obtained from Patrinia villosa by 9.0% NaOH extraction, was fractionated by anion-exchange and gel filtration chromatography. Using Fourier infrared spectrometry, gas chromatography, methylation, and NMR, we determined PVPS-3 to be a 2.61 kDa heteropolysaccharide composed of L-arabinose, D-mannose, D-glucose, and D-galactose in a ratio of 2.3:1.1:4.9:1.2. Purified PVPS-3 showed concentration-dependent scavenging activity of 2,2-diphenyl-1-picrylhydrazyl radical and moderate hydroxyl radical scavenging (IC50 1.5 mg/mL) compared with crude polysaccharide (IC50 2.3 mg/mL). PVPS-3 is promising as a potential antioxidant.
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Acknowledgment
This work was financially supported by the National Natural Science Foundation of China (No. 31571759), the Key Project of Science and Technology of Zhejiang (No. 2012C12004-4), the Basic Conditions of Local Science and Technology under the Central Government Subsidy (No. 20130611), the Analyzing and Testing Fund of Zhejiang (No. 2014C37106), and the State Forestry Administration, P. R. China (2008-4-64).
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Published in Khimiya Prirodnykh Soedinenii, No. 3, May–June, 2016, pp. 338–340.
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He, L., Han, S.F., Cheng, J.W. et al. Structural Characterization and Antioxidant Activity of a Novel Alkaline-Soluble Polysaccharide from Patrinia villosa . Chem Nat Compd 52, 384–387 (2016). https://doi.org/10.1007/s10600-016-1654-z
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DOI: https://doi.org/10.1007/s10600-016-1654-z