Abstract
To identify the putative interacting partners for Kiaa0319-like protein. KIAA0319-like, located near the dyslexia susceptibility locus, DYX8 in chromosome 1p34.3, has been suggested as a positional candidate for developmental dyslexia due to its homology with another gene, KIAA0319 which has been strongly established as a candidate gene for developmental dyslexia. Previous research has shown that a single marker, rs7523017 (P = 0.042) has been associated with developmental dyslexia by a Canadian group. There is little functional information about this gene and protein. In this article, we put forward further evidence that support Kiaa0319-like is a candidate for this disorder. A yeast-2-hybrid screen and co-immunopreciptiation assays were performed to find protein interacting partners of KIAA0319L. A human cortex immunohistochemistry assay was performed to show the colocalization of Kiaa0319-like and its specific interacting partner in cells. Nogo Receptor 1 (NgR1), an axon guidance receptor, was identified to have physical interactions with Kiaa0319-like protein. These two proteins interact predominantly in the cytoplasmic granules of cortical neurons in the human brain cortex. Based on this data, it can be concluded that Kiaa0319-like protein interacts with Nogo Receptor 1, supporting the idea that Kiaa0319-like protein participates in axon guidance.
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Acknowledgments
This study was partially supported by grants and a donation from the Croucher Foundation (to MMYW). MWP and WHT are the recipients of graduate studentships from the University Grants Council of Hong Kong.
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10571_2010_9549_MOESM1_ESM.ppt
Supplementary Fig. 1. Kiaa0319L protein did not self activate in the yeast two-hybrid system. Yeast strain AH109 was co-transformed with the bait plasmid, Kiaa0319L (N)-pGBK-BD and empty-pGAD-AD. No interaction was indicated by (A) β-galactosidase colorimetric filter assay and (B) SD/QDO culturing. (PPT 3647 kb)
10571_2010_9549_MOESM2_ESM.ppt
Supplementary Fig. 2. Schematic diagrams showing the predicted domains for human Kiaa0319 and Kiaa0319L proteins. Human Kiaa0319 protein sequence NP_055624.2 and human Kiaa0319L protein sequence NP_079150.3 are shown. (PPT 142 kb)
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Poon, MW., Tsang, WH., Chan, SO. et al. Dyslexia-Associated Kiaa0319-Like Protein Interacts with Axon Guidance Receptor Nogo Receptor 1. Cell Mol Neurobiol 31, 27–35 (2011). https://doi.org/10.1007/s10571-010-9549-1
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DOI: https://doi.org/10.1007/s10571-010-9549-1