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Looking at time dependent differentiation of mesenchymal stem cells by culture media using MALDI-TOF-MS

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Abstract

Mesenchymal stem cells (MSCs) are multipotent cells which are popular in human regenerative medicine. These cells can renew themselves and differentiate into several specialized cell types including osteoblasts, adipocytes, and chondrocytes under physiological and experimental conditions. MSCs can secret a lot of components including proteins and metabolites. These components have significant effects on their surrounding cells and also can be used to characterize them. This characterization of multipotent MSCs plays a critical role in their therapeutic potential. The metabolic profile of culture media verified by applying matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF-MS) techniques. Also, the differentiation and development of MSCs have monitored through culture media metabolome or secretome (secreted metabolites). Totally, 24 potential metabolites were identified. Between them 12 metabolites are unique to BM-MSCs and 5 metabolites are unique to AD-MSCs. Trilineage differentiation including chondrocytes, osteoblasts, and adipocytes, as well as metabolites that are being differentiated, have been shown in different weeks. In the present study, the therapeutic effects of MSCs analyzed by decoding the metabolome for MSCs secretome via metabolic profiling using MALDI-TOF-MS techniques.

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Abbreviations

MSCs:

Mesenchymal stem cells

MALDI-TOF-MS:

Matrix-assisted laser desorption and ionization time-of-flight mass spectrometry

PCA:

Principal component analysis

ISCT:

International society for cell therapy

HLA-DR:

Human leukocyte antigen—DR isotype

CD:

Cluster of differentiation

GVHD:

Graft versus host disease

DHB:

Dihydroxybenzoic acid

TFA:

Trifluoroacetic acid

MATLAB:

Matrix laboratory

hES cells:

Human embryonic stem cells

MS:

Mass spectrometry

METLIN:

Metabolite mass spectral database

HMDB:

Human metabolome database

KEGG:

Kyoto encyclopedia of genes and genomes

PLS-DA:

Partial least squares discriminant analysis

BM-MSCs:

Bone-marrow-derived mesenchymal stem cells

AD-MSCs:

Adipose-derived mesenchymal stem cells

m/z:

Mass-to-charge ratio

AD:

Alzheimer's disease

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Acknowledgements

The authors would like to acknowledge Dr. Mohsen Khorshidi and Shokouh Salimi for their kind support.

Author information

Authors and Affiliations

  1. Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

    Kambiz Gilany

  2. Integrative Oncology Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran

    Kambiz Gilany

  3. Metabolomics and Genomics Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

    Parisa Goodarzi & Babak Arjmand

  4. Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

    Akram Tayanloo-Beik & Babak Arjmand

  5. Chemometrics Laboratory, Department of Chemistry, Tarbiat modares university, 14115-111, Tehran, Iran

    Mohammad Javad Masroor

  6. Chemometrics Laboratory, Department of Chemistry, Tarbiat Modares University, Tehran, Iran

    Ahmad Mani-Varnosfaderani

  7. Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Mostafa Rezaei-Tavirani

  8. Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Molecular-Cellular. Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

    Hamidreza Aghayan

  9. Sports Medicine Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran

    Ramin Kordi

  10. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

    Bagher Larijani

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  1. Kambiz Gilany
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  2. Parisa Goodarzi
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  3. Akram Tayanloo-Beik
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  7. Hamidreza Aghayan
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  8. Ramin Kordi
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  9. Babak Arjmand
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Contributions

KG supervised the study design, experiment, and manuscript writing. PG, MR participated in critical review of the manuscript. RK participated in the study design and interpretation. AT manuscript writing and participated in statistical data analysis. HA manuscript writing and participated in the study design. MM, AM performed statistical data analysis and analyzed the raw data and performed the metabolite identification. BL supervised the project from the scientific view of point and advised on experimental design. BA supervised and designed the experiment and manuscript writing and all authors reviewed the manuscript.

Corresponding authors

Correspondence to Babak Arjmand or Bagher Larijani.

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Gilany, K., Goodarzi, P., Tayanloo-Beik, A. et al. Looking at time dependent differentiation of mesenchymal stem cells by culture media using MALDI-TOF-MS. Cell Tissue Bank 23, 653–668 (2022). https://doi.org/10.1007/s10561-021-09963-3

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  • DOI: https://doi.org/10.1007/s10561-021-09963-3

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