Abstract
To compare ex vivo results of donor corneas maintained in Sinasol with those stored in Optisol-GS and reporting clinical outcomes of grafted Sinasol-versus Optisol-GS-stored corneas. In phase I, paired donor corneas were maintained in Sinasol or Optisol-GS. Afterward, the corneas were subjected to slit-lamp biomicroscopic and specular microscopic examinations on days 1 and 7, and then to trypan blue staining on day 7. The same examinations were performed on the corneas that were kept in Sinasol or Optisol-GS for 14 days. In phase II, the post-operative reports of 72 consecutive corneal transplantations were recorded using Sinasol- or Optisol-GS-preserved corneas. In phase I, 128 corneas from 64 donors and 59 corneas from 33 donors were investigated for 7 and 14 days, respectively. The EC indices were comparable between the groups at the measurement periods. The EC losses over 7 and 14 days were 3.7% and 19.9% in Sinasol against 4.6% and 20.8% in Optisol-GS. Although fair quality corneas were more common in Optisol-GS group after 7 (P = 0.04) and 14 days (P = 0.034), changes of stromal edema, Descemet’s fold, and other quality ratings during 14 days were not different between the groups. In phase II, all the transplanted corneas were postoperatively clear with no adverse reactions. The overall results indicate that Sinasol is a safe, effective, and affordable intermediate cold storage medium for preservation of corneas.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Basak S, Prajna NV (2016) A prospective, in vitro, randomized study to compare two media for donor corneal storage. Cornea 35:1151–1155
de Belder AN (2003) Dextran, 2nd edn. Little Chalfont, Armersham Biosciences, p 12, 31
European Eye Bank Association (EEBA) Standards (2020) Technical guidelines for ocular tissue, revision 11. https://www.eeba.eu/files/pdf/EEBA_Technical_Guidelines_for_Ocular_Tissue_Revision11.pdf
Feizi S (2016) Donor graft quality used for penetrating keratoplasty and deep anterior lamellar keratoplasty. In: Pacheco P (ed) Advances in eye surgery. IntechOpen, London, pp 859–973 (Chapter 4)
Feizi S, Javadi MA, Kanavi MR, Javadi F (2014) Effect of donor graft quality on clinical outcomes after deep anterior lamellar keratoplasty. Cornea 33:795–800
Jardine GJ, Holiman JD, Stoeger CG, Chamberlain WD (2014) Imaging and quantification of endothelial cell loss in eye bank prepared DMEK grafts using trainable segmentation software. Curr Eye Res 39:894–901
Jeng BH (2006) Preserving the cornea: corneal storage media. Curr Opin Ophthalmol 17:332–337
Kanavi MR, Javadi MA, Chamani T, Fahim P, Javadi F (2015) Comparing quantitative and qualitative indices of the donated corneas maintained in Optisol-GS with those kept in Eusol-C. Cell Tissue Bank 16:243–247
Medical Standards (2013) The Eye Bank Association of America (EBAA). http://restoresight.org/wp-content/uploads/2014/01/Medical-Standards-November-2013.pdf
Møller-Pedersen T, Hartmann U, Møller HJ, Ehlers N, Engelmann K (2001) Evaluation of potential organ culture media for eye banking using human donor corneas. Br J Ophthalmol 85:1075–1079
Nelson LR, Hodge DO, Bourne WM (2000) In vitro comparison of Chen medium and Optisol-GS medium for human corneal storage. Cornea 19:782–787
Parekh M, Salvalaio G, Ferrari S, Amoureux MC, Albrecht C, Fortier D, Ponzin D (2014) A quantitative method to evaluate the donor corneal tissue quality used in a comparative study between two hypothermic preservation media. Cell Tissue Bank 15:543–554
Parekh M, Ferrari S, Salvalaio G, Ponzin D (2015) Synthetic versus serum-based medium for corneal preservation in organ culture: a comparative study between 2 different media. Eur J Ophthalmol 25:96–100
Patel Dh (2017) Eye banking. In: I notes ophthalmology PG exam notes, cornea (eBook), 1st edn. 'DB' Da Books, p 27
Pels E, Rijneveld WJ (2009) Organ culture preservation for corneal tissue: technical and quality aspects. Dev Ophthalmol 43:31–46
Pham C, Hellier E, Vo M, Szczotka-Flynn L (2013) Donor endothelial specular image quality in Optisol GS and Life4˚C. Int J Eye Bank 1:1–8
Sperling S (1986) Evaluation of the endothelium of human donor corneas by induced dilation of intercellular spaces and trypan blue. Graefes Arch Clin Exp Ophthalmol 224:428–434
Wilson SE, Bourne WM (1989) Corneal preservation. Surv Ophthalmol 33:237–259
Acknowledgements
The authors would like to thank the processing laboratory technicians of the Central Eye Bank of Iran for their help during the ex vivo investigations.
Funding
None.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Ethical approval
The ex vivo and the clinical protocols were reviewed and approved by the Institutional Review Board of the Central Eye Bank of Iran and the ethics committee of the Ophthalmic Research Center affiliated with the Shahid Beheshti University of Medical Sciences, Tehran-Iran. Additionally, signed informed consent was obtained from the cornea recipients enrolled in the second phase of the study in terms of the principles of the Declaration of Helsinki.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Javadi, M.A., Rezaeian Akbarzadeh, A., Chamani, T. et al. Sinasol versus Optisol-GS for cold preservation of human cornea: a prospective ex vivo and clinical study. Cell Tissue Bank 22, 563–574 (2021). https://doi.org/10.1007/s10561-021-09930-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10561-021-09930-y