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Using paracrine effects of Ad-MSCs on keratinocyte cultivation and fabrication of epidermal sheets for improving clinical applications

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Abstract

Recent advances in wound healing have made cell therapy a potential approach for the treatment of various types of skin defects such as trauma, burns, scars and diabetic leg ulcers. Cultured keratinocytes have been applied to burn patients since 1981. Patients with acute and chronic wounds can be treated with autologous/allograft cultured keratinocytes. There are various methods for cultivation of epidermal keratinocytes used in cell therapy. One of the important properties of an efficient cell therapy is the preservation of epidermal stem cells. Mesenchymal Stem Cells (MSCs) are major regulatory cells involved in the acceleration of wound healing via induction of cell proliferation, angiogenesis and stimulating the release of paracrine signaling molecules. Considering the beneficial effects of MSCs on wound healing, the main aim of the present study is investigating paracrine effects of Adipose-derived Mesenchymal Stem Cell (Ad-MSCs) on cultivation of keratinocytes with focusing on preservation of stem cells and their differentiation process. We further introduced a new approach for culturing isolated keratinocytes in vitro in order to generate epidermal keratinocyte sheets without using a feeder layer. To do so, Ad-MSC conditioned medium was applied as an alternative to commercial media for keratinocyte cultivation. In this study, the expression of several stem/progenitor cell (P63, K19 and K14) and differentition (K10, IVL and FLG) markers was examined using real time PCR on days 7, 14 and 21 of culture in keratinocytes in Ad-MSC conditioned medium. P63 and α6 integrin expression was also evaluated via flow cytometry. The results were compared with control group including keratinocytes cultured in EpiLife medium and our data indicated that this Ad-MSC conditioned medium is a good alternative for keratinocyte cultivation and producing epidermal sheets for therapeutic and clinical purposes. The reasons are the expression of stem cell and differentiation markers and overcoming the requirement for feeder layer which leads to a xenograft-free transplantation. Besides, this approach has low cost and is easier to perform. However, more in vitro and in vivo experiments as well as safety evaluation required before clinical applications.

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Acknowledgement

The authors are grateful to Sonia Iranpour, Mahboubeh Kazemi and Asieh Heirani-Tabasi for their technical assistance.

Funding

This study was funded by a Grant from ACECR-Khorasan Razavi Branch (12.987).

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Authors and Affiliations

  1. Stem Cell and Regenerative Medicine Research Group, Iranian Academic Center for Education, Culture and Research (ACECR), Khorasan Razavi Branch, Mashhad, Iran

    Halimeh Hassanzadeh, Maryam M. Matin, Hojjat Naderi-Meshkin, Hamid Reza Bidkhori, Mahdi Mirahmadi, Mahmood Raeesolmohaddeseen & Ahmad Reza Bahrami

  2. Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran

    Halimeh Hassanzadeh, Maryam M. Matin & Ahmad Reza Bahrami

  3. Novel Diagnostics and Therapeutics Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran

    Maryam M. Matin & Ahmad Reza Bahrami

  4. Department of General Surgery, Islamic Azad University Mashhad Branch, Mashhad, Iran

    Nasser Sanjar-Moussavi

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  1. Halimeh Hassanzadeh
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  2. Maryam M. Matin
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  3. Hojjat Naderi-Meshkin
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  4. Hamid Reza Bidkhori
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  7. Nasser Sanjar-Moussavi
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  8. Ahmad Reza Bahrami
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Correspondence to Maryam M. Matin or Ahmad Reza Bahrami.

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Hassanzadeh, H., Matin, M.M., Naderi-Meshkin, H. et al. Using paracrine effects of Ad-MSCs on keratinocyte cultivation and fabrication of epidermal sheets for improving clinical applications. Cell Tissue Bank 19, 531–547 (2018). https://doi.org/10.1007/s10561-018-9702-5

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