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Dietary aflatoxin B1 intake, genetic polymorphisms of CYP1A2, CYP2E1, EPHX1, GSTM1, and GSTT1, and gastric cancer risk in Korean

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Abstract

Purpose

We investigated the effects of aflatoxin B1 (AFB1) intake, genetic polymorphisms of AFB1 metabolic enzymes, and interactions between the polymorphisms and intake of AFB1 with regard to the risk of gastric cancer in Korean.

Methods

The participants in the study included 477 gastric cancer patients and 477 age- and sex-matched control subjects. Direct interviews and a structured questionnaire were used to determine the level of exposure to AFB1, and the GoldenGate assay and multiplex polymerase chain reaction were used for genotypic analyses of the cytochrome P450 1A2 (CYP1A2), cytochrome P450 1E1, epoxide hydrolase 1, and glutathione S-transferase genes.

Results

The probable daily intake of AFB1 was significantly higher among gastric cancer patients than among control subjects (cases vs. controls: 1.91 ± 0.87 vs. 1.65 ± 0.72 ng/kg bw/day, p < 0.0001), and increased AFB1 intake was significantly associated with an elevated risk of gastric cancer (odds ratio 1.94; 95 % confidence interval 1.43–2.63). However, genetic polymorphisms of AFB1 metabolic enzymes were not associated with gastric cancer, with the exception of CYP1A2. Moreover, there was no interaction between AFB1 intake and the genotypes of metabolic enzymes that affect gastric cancer risk.

Conclusions

Our results suggest that dietary AFB1 exposure might be associated with a risk of gastric cancer. However, the effect of AFB1 on gastric carcinogenesis may not be modulated by genetic polymorphisms of AFB1 metabolic enzymes.

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Acknowledgments

This study was supported by a grant from the National R&D Program for Cancer Control, Ministry of Health & Welfare, Republic of Korea (1120330).

Conflict of interest

None.

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Authors and Affiliations

  1. Department of Preventive Medicine and Medical Research Institute, College of Medicine, Chungbuk National University, 52 Naesudong-ro, Heungdok-gu, Cheongju, 361-763, Korea

    Sang-Yong Eom, Dong-Hyuk Yim, Yanwei Zhang, Jung-Kuk Yun, Sun In Moon, Yong-Dae Kim & Heon Kim

  2. Department of Surgery, College of Medicine, Chungbuk National University, Cheongju, Korea

    Hyo-Yung Yun & Young-Jin Song

  3. Department of Internal Medicine, College of Medicine, Chungbuk National University, Cheongju, Korea

    Sei-Jin Youn

  4. Department of Food and Nutrition, Chungbuk National University, Cheongju, Korea

    Taisun Hyun

  5. Department of Surgery, College of Medicine, Eulji University, Daejon, Korea

    Joo-Seung Park

  6. Department of Surgery, Asan Medical Center, College of Medicine, Ulsan University, Seoul, Korea

    Byung Sik Kim

  7. Center for Genome Science, National Institute of Health, Osong, Korea

    Jong-Young Lee

Authors
  1. Sang-Yong Eom
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  2. Dong-Hyuk Yim
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  14. Heon Kim
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Correspondence to Heon Kim.

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Eom, SY., Yim, DH., Zhang, Y. et al. Dietary aflatoxin B1 intake, genetic polymorphisms of CYP1A2, CYP2E1, EPHX1, GSTM1, and GSTT1, and gastric cancer risk in Korean. Cancer Causes Control 24, 1963–1972 (2013). https://doi.org/10.1007/s10552-013-0272-3

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