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Silicon membrane filter designed by fluid dynamics simulation and near-field stress analysis for selective cell enrichment

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Abstract

Selective cell enrichment technologies can play an important role in both diagnostic and therapeutic areas. However, currently used cell sorting techniques have difficulties in rapidly isolating only the desired target cells from a large volume of body fluids. In this work, we developed a filtering system that can quickly separate and highly concentrate cells from a large volume of solution, depending on their size, using a silicon membrane filter. To overcome the problems caused by material limitations of the brittle silicon, we designed a novel membrane filter with various pore designs. From these designs, the most optimal design with high pore density, while preventing crack formation was derived by applying fluid dynamics simulation and near-field stress analysis. The membrane filter system using the selected design was fabricated, and cell filtration performance was evaluated. The LNCaP cell in horse blood was recovered up to 86% and enriched to 187-fold compared to initial cell populations after filtration at a flow rate of 5 mL/min. The results demonstrate that the filter presented in this study can rapidly and selectively isolate target cells from a large volume of body fluid sample.

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Acknowledgments

This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Ministry of Science, ICT (2017M3A9E4063814), and the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health & Welfare, Republic of Korea (HI14C3229).

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Author notes

  1. Yo-Chang Jang and Hyun-Ju Park contributed equally to this work.

Authors and Affiliations

  1. Medical Device Management and Research, Samsung Advanced Institute of Health Sciences and Technology, Sungkyunkwan University, Seoul, 06351, South Korea

    Yo-Chang Jang, Hyun-Ju Park, Ayoung Woo, Kyu-Sung Lee & Min-Young Lee

  2. Smart Healthcare Medical Device Research Center, Samsung Medical Center, 81, Irwon-ro, Gangnam-gu, Seoul, 06351, South Korea

    Kyu-Sung Lee & Min-Young Lee

  3. Samsung Genome Institute, Samsung Medical Center, 81, Irwon-ro, Gangnam-gu, Seoul, 06351, South Korea

    Hui-Sung Moon

  4. Samsung Electronics, Samsung Biomedical Research Institute, Samsung Advanced Institute of Technology, Seoul, 06351, South Korea

    Jin Ho Oh

Authors
  1. Yo-Chang Jang
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  2. Hyun-Ju Park
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  3. Ayoung Woo
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  4. Kyu-Sung Lee
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  5. Hui-Sung Moon
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Corresponding authors

Correspondence to Hui-Sung Moon, Jin Ho Oh or Min-Young Lee.

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Jang, YC., Park, HJ., Woo, A. et al. Silicon membrane filter designed by fluid dynamics simulation and near-field stress analysis for selective cell enrichment. Biomed Microdevices 20, 87 (2018). https://doi.org/10.1007/s10544-018-0334-0

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