Abstract
We present a new strategy for fabricating a silicon nanopore device allowing straightforward fluidic integration and electrical as well as optical monitoring. The device presents nanopores of diameters 10 nm to 160 nm, and could therefore be used to obtain solvent-free free-standing lipid bilayers from small unilamellar vesicles (SUV) or large unilamellar vesicles (LUV). The silicon chip fabrication process only requires front side processing of a silicon-on-insulator (SOI) substrate. A polydimethylsiloxane (PDMS) microfluidic interface is assembled on the silicon chip for fluidic handling and electrical addressing. We detail the electrical specifications of our device and some perspectives showing that the use of an SOI substrate is a convenient way to reduce the electrical noise in a silicon nanopore device without the need of a photolitographic patterned passivation layer. We then demonstrate simultaneous electrical and optical monitoring by capturing negatively charged fluorescent nanoparticles. Finally, in the perspective of solvent-free free-standing lipid bilayers, we show that incubation of SUV results in a drastic increase of the device electrical resistance, which is likely due to the formation of a free-standing lipid bilayer sealing the nanopores.
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Notes
Unilamellar vesicles are vesicles made by a single phospholipid bilayer enclosing a volume of aqueous solution.
1-acyl-2-{12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino] dodecanoyl}-sn-glycero-3-phosphocholine
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine
The content of reservoir 4 is removed and replaced by PBS buffer. The same procedure is repeated for reservoir 2.
see note 3
see note 2
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Acknowledgements
We thank Serge Mazères and Laurence Salomé from IPBS (Toulouse, France) for their kind collaboration on FRAP experiments. We also thank A. Lecestre, P. Dubreuil and B. Rousset for their involvement in the fabrication of the silicon chips. This work was partly supported by LAAS-CNRS micro and nanotechnologies facilities platform member of the French RENATECH network.
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Marchand, R., Thibault, C., Carcenac, F. et al. Integration of solid-state nanopores into a functional device designed for electrical and optical cross-monitoring. Biomed Microdevices 19, 60 (2017). https://doi.org/10.1007/s10544-017-0195-y
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DOI: https://doi.org/10.1007/s10544-017-0195-y