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A new phosphothreonine lyase electrochemical immunosensor for detecting Salmonella based on horseradish peroxidase/GNPs-thionine/chitosan

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Abstract

In the current study, a novel double-layer gold nanoparticles- electrochemical immunosensor electrode (DGN-EIE) immobilized with Salmonella plasmid virulence C (SpvC) antibody was developed. To increase the fixed quantity of antibodies and electrochemical signal, an electrochemical biosensing signal amplification system was utilized with gold nanoparticles-thionine-chitosan absorbing horseradish peroxidase (HRP). In addition, the SpvC monoclonal antibodies (derived from Balb/c mice) were prepared and screened with a high affinity to SpvC. To evaluate the quality of DGN-EIE, the amperometric I-t curve method was applied to determine Salmonella in PBS. The results showed that the response current had a good linear correlation with the bacterial quantity ranged from 1.0 × 101–5.0 × 104 cfu/mL. The lowest detection limit was found at 5 cfu/mL. Furthermore, the proposed immunosensor has been demonstrated with high sensitivity, good selectivity and reproducibility. Apparently, DGN-EIE may be a very useful tool for monitoring the bacteria.

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Acknowledgements

The work was supported by the National Natural Science Foundation of China (No. 31671857 and No. 31371773). In addition, we appreciate Shao-Feng lab for supplying SpvC recombinant plasmid.

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Authors and Affiliations

  1. Biotechnology & Food Science College, Tianjin University of Commerce, Tianjin, 300314, China

    Dingqiang Lu, Guangchang Pang & Junbo Xie

  2. Tianjin Key Laboratory of Food Biotechnology, Tianjin, 300314, China

    Guangchang Pang & Junbo Xie

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  1. Dingqiang Lu
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  2. Guangchang Pang
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  3. Junbo Xie
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Correspondence to Guangchang Pang or Junbo Xie.

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Lu, D., Pang, G. & Xie, J. A new phosphothreonine lyase electrochemical immunosensor for detecting Salmonella based on horseradish peroxidase/GNPs-thionine/chitosan. Biomed Microdevices 19, 12 (2017). https://doi.org/10.1007/s10544-017-0149-4

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