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Identification and expression analysis of nuclear factor Y families in Prunus mume under different abiotic stresses

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Biologia Plantarum

Abstract

The nuclear factor Y (NF-Y) is one of the largest transcription factor families in plants consisting of NF-YA, NF-YB, and NF-YC subunits. It could play important roles in various processes such as flowering time, seed development, and response to drought. In this study, 6 NF-YA, 13 NF-YB, and 8 NF-YC proteins were identified and characterized in Prunus mume. Analyses of a conserved domain indicated that the PmNF-Y subunits shared an elevated degree of homology with the corresponding Arabidopsis NF-Y ones. Phylogenetic analysis showed that each NF-Y subunit family from Prunus mume and Arabidopsis could be divided into 4 or 2 clades based on their full-length proteins. The gene expression patterns of all 27 PmNF-Y genes were examined under abscisic acid (ABA), osmotic, salt, and H2O2 treatments using real-time quantitative PCR analyses. PmNF-YA1/2/4/5/6, PmNF-YB3/4/8/10/11/13, and PmNF-YC1/2/4/5/6/8 were found to be up-regulated under the ABA and osmotic treatments. PmNF-YA1/2/3/4/5/6, PmNF-YB1/3/8/10/11/13, and PmNF-YC1/2/5/6/8 were obviously induced by the H2O2. In addition, only PmNF-YA2 and PmNF-YB3 expressions were enhanced under the salt stress. These findings could provide an entry point to investigating the roles of PmNF-Y genes during abiotic stress responses.

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Abbreviations

ABA:

abscisic acid

CBF:

CCAAT-binding factor

CDS:

coding sequence

HAP:

heme activator protein

NF-Y:

nuclear factor Y

ORF:

open reading frame

qPCR:

quantitative PCR

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Authors and Affiliations

  1. Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, P.R. China

    J. Yang, X. L. Wan, C. Guo, J. W. Zhang & M. Z. Bao

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  1. J. Yang
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  2. X. L. Wan
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  3. C. Guo
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  4. J. W. Zhang
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  5. M. Z. Bao
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Correspondence to J. W. Zhang.

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Acknowledgement

This research was financially supported by the National Natural Science Foundation of China (grant No. 31270739) and the National 863 Project of China (No. 2011AA100207).

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Yang, J., Wan, X.L., Guo, C. et al. Identification and expression analysis of nuclear factor Y families in Prunus mume under different abiotic stresses. Biol Plant 60, 419–426 (2016). https://doi.org/10.1007/s10535-016-0624-4

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  • DOI: https://doi.org/10.1007/s10535-016-0624-4

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