Abstract
To investigate the effect of Selenium Rich Yeast (SeY) on hepatotoxicity of Aluminium (Al), SeY (0.1 mg/kg) was orally administrated to aluminium-exposed mice (10 mg/kg) for 28 days. The risk of oxidative stress was assessed by detecting the total antioxidant capacity (T-AOC), catalase activity, H2O2 content, and mRNA levels of the Keap1/Nrf-2/HO-1 pathway. Inflammatory reactions were assessed by detecting the mRNA levels of inflammatory biomarkers. Our results showed that SeY protected against the liver histological changes induce by Al. The body weight gain of mice treated with SeY + Al restore to normal compare with mice exposed to Al alone. Al treatment significantly decreased the activities of antioxidant enzymes, reduced T-AOC levels, and up-regulated the mRNA level of Nrf2 and HO-1, thereby ultimately leading to peroxidation. SeY shown a significant protective effect against oxidative stress caused by Al. In addition, Al exposure induced inflammatory responses in rat liver by promoting the release of inflammatory cytokines (TNF-a, NF-kB, TNF-R1, IL-1, IL-6, and COX-2). SeY protected against changes in liver by regulating the mRNA expression levels of inflammatory factors. These results suggested that Se protected the liver from the Al-induced hepatotoxicity by regulating the mRNA level of Keap1/Nrf2/HO-1, and inhibited inflammatory responses by down-regulating the expression level of inflammatory cytokine.
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References
Bansal MP, Kaur P (2005) Selenium, a versatile trace element: current research implications. Indian J Exp Biol 43:1119–1129
Brenneisen P, Steinbrenner H, Sies H (2005) Selenium, oxidative stress, and health aspects. Mol Asp Med 26:256–267. https://doi.org/10.1016/j.mam.2005.07.004
Brigelius-Flohe R, Flohe L (2003) Is there a role of glutathione peroxidases in signaling and differentiation? BioFactors 17:93–102
Celi P, Gabai G (2015) Oxidant/antioxidant balance in animal nutrition and health: the role of protein oxidation frontiers in veterinary science 2:48. https://doi.org/10.3389/fvets.2015.00048
Cheng D, Zhang X, Xu L, Li X, Hou L, Wang C (2017) Protective and prophylactic effects of chlorogenic acid on aluminum-induced acute hepatotoxicity and hematotoxicity in mice. Chem Biol Interact 273:125–132. https://doi.org/10.1016/j.cbi.2017.06.013
Deshmukh P, Unni S, Krishnappa G, Padmanabhan B (2017) The Keap1-Nrf2 pathway: promising therapeutic target to counteract ROS-mediated damage in cancers and neurodegenerative diseases. Biophys Rev 9:41–56. https://doi.org/10.1007/s12551-016-0244-4
El-Demerdash FM (2004) Antioxidant effect of vitamin E and selenium on lipid peroxidation, enzyme activities and biochemical parameters in rats exposed to aluminium. J Trace Elem Med Biol 18:113–121. https://doi.org/10.1016/j.jtemb.2004.04.001
Exley C (2016) The toxicity of aluminium in humans. Morphologie 100:51–55. https://doi.org/10.1016/j.morpho.2015.12.003
Garcia-Nino WR, Pedraza-Chaverri J (2014) Protective effect of curcumin against heavy metals-induced liver damage. Food Chem Toxicol 69:182–201. https://doi.org/10.1016/j.fct.2014.04.016
Ghorbel I, Maktouf S, Kallel C, Ellouze Chaabouni S, Boudawara T, Zeghal N (2015) Disruption of erythrocyte antioxidant defense system, hematological parameters, induction of pro-inflammatory cytokines and DNA damage in liver of co-exposed rats to aluminium and acrylamide. Chem Biol Interact 236:31–40. https://doi.org/10.1016/j.cbi.2015.04.020
Ghorbel I et al (2016) Expression of metallothioneins I and II related to oxidative stress in the liver of aluminium-treated rats. Arch Physiol Biochem 122:214–222. https://doi.org/10.1080/13813455.2016.1187176
Gouda AS, El-Nabarawy NA, Ibrahim SF (2018) Moringa oleifera extract (Lam) attenuates Aluminium phosphide-induced acute cardiac toxicity in rats. Toxicol Rep 5:209–212. https://doi.org/10.1016/j.toxrep.2018.01.001
Guo CH, Hsu GS, Chuang CJ, Chen PC (2009) Aluminum accumulation induced testicular oxidative stress and altered selenium metabolism in mice. Environ Toxicol Pharmacol 27:176–181. https://doi.org/10.1016/j.etap.2008.10.001
Hong C et al (2017) The Chinese herbal formula Free and Easy Wanderer ameliorates oxidative stress through KEAP1-NRF2/HO-1 pathway. Sci Rep 7:11551. https://doi.org/10.1038/s41598-017-10443-6
Hou S et al (2018) S100A4 protects mice from high-fat diet-induced obesity and inflammation. Lab Investig. https://doi.org/10.1038/s41374-018-0067-y
Krohn RM, Lemaire M, Negro Silva LF, Lemarie C, Bolt A, Mann KK, Smits JE (2016) High-selenium lentil diet protects against arsenic-induced atherosclerosis in a mouse model. J Nutr Biochem 27:9–15. https://doi.org/10.1016/j.jnutbio.2015.07.003
Kubachka KM et al (2017) Evaluation of selenium in dietary supplements using elemental speciation. Food Chem 218:313–320. https://doi.org/10.1016/j.foodchem.2016.08.086
Lakshmi BV, Sudhakar M, Prakash KS (2015) Protective effect of selenium against aluminum chloride-induced Alzheimer’s disease: behavioral and biochemical alterations in rats. Biol Trace Elem Res 165:67–74. https://doi.org/10.1007/s12011-015-0229-3
Li X et al (2017) Effects of selenium-lead interaction on the gene expression of inflammatory factors and selenoproteins in chicken neutrophils. Ecotoxicol Environ Saf 139:447–453. https://doi.org/10.1016/j.ecoenv.2017.02.017
Liu J (2014) Ethanol and liver: recent insights into the mechanisms of ethanol-induced fatty liver. World J Gastroenterol 20:14672–14685. https://doi.org/10.3748/wjg.v20.i40.14672
Liu S, Xu F, Fu J, Li S (2015) Protective roles of selenium on nitric oxide and the gene expression of inflammatory cytokines induced by cadmium in chicken splenic lymphocytes. Biol Trace Elem Res 168:252–260. https://doi.org/10.1007/s12011-015-0354-z
Molnar J (2013) Selenium: its antioxidant effects and issues in selenium supply. Orv Hetil 154:1613–1619. https://doi.org/10.1556/oh.2013.29705
Moustafa T et al (2012) Alterations in lipid metabolism mediate inflammation, fibrosis, and proliferation in a mouse model of chronic cholestatic liver injury. Gastroenterology 142:140–151. https://doi.org/10.1053/j.gastro.2011.09.051
Mundugaru R, Sivanesan S, Udaykumar P, Rao N, Chandra N (2017) Protective effect of Pluchea lanceolata against aluminum chloride-induced neurotoxicity in Swiss albino mice. Pharmacogn Mag 13:S567–S572. https://doi.org/10.4103/pm.pm_124_17
Nattrass C, Horwell CJ, Damby DE, Brown D, Stone V (2017) The effect of aluminium and sodium impurities on the in vitro toxicity and pro-inflammatory potential of cristobalite. Environ Res 159:164–175. https://doi.org/10.1016/j.envres.2017.07.054
Nettleford SK, Prabhu KS (2018) Selenium and selenoproteins in gut inflammation—a review. Antioxidants 7:36. https://doi.org/10.3390/antiox7030036
Olteanu D et al (2012) The effects of chitosan and low dose dexamethasone on extrahepatic cholestasis after bile duct ligation in Wistar rats. Acta Physiol Hung 99:61–73. https://doi.org/10.1556/APhysiol.99.2012.1.7
Park CY, Park S, Kim MS, Kim HK, Han SN (2017) Effects of mild calorie restriction on lipid metabolism and inflammation in liver and adipose tissue. Biochem Biophys Res Commun 490:636–642. https://doi.org/10.1016/j.bbrc.2017.06.090
Pineton de Chambrun G et al (2014) Aluminum enhances inflammation and decreases mucosal healing in experimental colitis in mice. Mucosal Immunol 7:589–601. https://doi.org/10.1038/mi.2013.78
Reszka E, Wieczorek E, Jablonska E, Janasik B, Fendler W, Wasowicz W (2015) Association between plasma selenium level and NRF2 target genes expression in humans. J Trace Elem Med Biol 30:102–106. https://doi.org/10.1016/j.jtemb.2014.11.008
Sanchez-Iglesias S, Mendez-Alvarez E, Iglesias-Gonzalez J, Munoz-Patino A, Sanchez-Sellero I, Labandeira-Garcia JL, Soto-Otero R (2009) Brain oxidative stress and selective behaviour of aluminium in specific areas of rat brain: potential effects in a 6-OHDA-induced model of Parkinson’s disease. J Neurochem 109:879–888
Stupin A et al (2017) Reduced dietary selenium impairs vascular function by increasing oxidative stress in Sprague-Dawley rat aortas. Int J Environ Res Public Health 14:591. https://doi.org/10.3390/ijerph14060591
Taguchi K, Motohashi H, Yamamoto M (2011) Molecular mechanisms of the Keap1-Nrf2 pathway in stress response and cancer evolution. Genes Cells 16:123–140. https://doi.org/10.1111/j.1365-2443.2010.01473.x
Thomas E, Yoneda M, Schiff ER (2015) Viral hepatitis: past and future of HBV and HDV. Cold Spring Harbor Perspect Med 5:a021345. https://doi.org/10.1101/cshperspect.a021345
Valente MJ, Carvalho F, Bastos M, de Pinho PG, Carvalho M (2012) Contribution of oxidative metabolism to cocaine-induced liver and kidney damage. Curr Med Chem 19:5601–5606
Yang X et al (2018) Bone impairment caused by AlCl3 is associated with activation of the JNK apoptotic pathway mediated by oxidative stress. Food Chem Toxicol 116:307–314. https://doi.org/10.1016/j.fct.2018.04.057
Yu L, Jiang R, Su Q, Yu H, Yang J (2014) Hippocampal neuronal metal ion imbalance related oxidative stress in a rat model of chronic aluminum exposure and neuroprotection of meloxicam. Behav Brain Funct 10:6. https://doi.org/10.1186/1744-9081-10-6
Zachara BA (2015) Selenium and selenium-dependent antioxidants in chronic kidney disease. Adv Clin Chem 68:131–151. https://doi.org/10.1016/bs.acc.2014.11.006
Zahedi-Amiri Z, Taravati A, Hejazian LB (2018) Protective effect of Rosa damascena against aluminum chloride-induced oxidative stress. Biol Trace Elem Res. https://doi.org/10.1007/s12011-018-1348-4
Zhang ZW, Zhang JL, Gao YH, Wang QH, Li S, Wang XL, Xu SW (2013) Effect of oxygen free radicals and nitric oxide on apoptosis of immune organ induced by selenium deficiency in chickens. Biometals 26:355–365. https://doi.org/10.1007/s10534-013-9612-8
Zheng W, Wang Q, Lu X, Shi Q, Zou J, Tao Y, Wang P (2016) Protective effects of Dracocephalum heterophyllum in ConA-Induced acute hepatitis. Mediat inflamm 2016:2684321. https://doi.org/10.1155/2016/2684321
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This work was supported by Foshan University research star-up project (099/gg07037) and Key Laboratory of preventive veterinary medicine in Guangdong Provincial Department of Education (2014KTSPT037).
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Luo, J., Li, X., Li, X. et al. Selenium-Rich Yeast protects against aluminum-induced peroxidation of lipide and inflammation in mice liver. Biometals 31, 1051–1059 (2018). https://doi.org/10.1007/s10534-018-0150-2
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DOI: https://doi.org/10.1007/s10534-018-0150-2