Abstract
The effects of changes in macrophage iron status, induced by single or multiple iron injections, iron depletion or pregnancy, on both immune function and mRNA expression of genes involved in iron influx and egress have been evaluated. Macrophages isolated from iron deficient rats, or pregnant rats at day 21 of gestation, either supplemented with a single dose of iron dextran, 10 mg, at the commencement of pregnancy, or not, showed significant increases of macrophage ferroportin mRNA expression, which was paralleled by significant decreases in hepatic Hamp mRNA expression. IRP activity in macrophages was not significantly altered by iron status or the inducement of pregnancy ± a single iron supplement. Macrophage immune function was significantly altered by iron supplementation and pregnancy. Iron supplementation, alone or combined with pregnancy, increased the activities of both NADPH oxidase and nuclear factor kappa B (NFκB). In contrast, the imposition of pregnancy reduced the ability of these parameters to respond to an inflammatory stimuli. Increasing iron status, if only marginally, will reduce the ability of macrophages to mount a sustained response to inflammation as well as altering iron homeostatic mechanisms.
Similar content being viewed by others
Abbreviations
- NO:
-
Nitric oxide
- DcytB:
-
Duodenal cytochrome B
- DMEM:
-
Dubecco’s modified eagle medium
- DMT1:
-
Divalent metal-ion transporter 1
- EDTA:
-
Ethylenediamine tetra acetate
- fmlp:
-
N formyl methionyl leucyl phenylalanine
- HEPES:
-
N-2-hydroxylethylpiperazine
- ICP-AES:
-
Inductively coupled plasma-atomic emission spectroscopy
- IRP:
-
Iron regulatory protein
- IRE:
-
Iron regulatory element
- IFNγ:
-
Interferon gamma
- LNMA:
-
NG-methyl-l-arginine
- LPS:
-
Lipopolysaccharide
- NFκB:
-
Nuclear factor kappa B
- NOS:
-
Nitric oxide synthase
- PMA:
-
Phorbol-12 myristate-13 acetate
- RT-PCR:
-
Reverse transcriptase polymerase chain reaction
- TIBC:
-
Total iron binding capacity
- TfR1:
-
Transferrin receptor 1
- TNFα:
-
Tumor necrosis factor alpha
References
Batey RG, Gallagher ND (1977) Role of the placenta in intestinal absorption of iron in pregnant rats. Gastroenterol 72:255–257
Beauchamp C, Fridovich I (1971) Superoxide dismutase improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287. doi:10.1016/0003-2697(71)90370-8
Bowie LA, O’Neill LA (2000) Oxidative stress and nuclear factor-kappaB activation: a reassessment of the evidence in the light of recent discoveries. Biochem Pharmacol 59:13–23. doi:10.1016/S0006-2952(99)00296-8
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye-binding. Anal Biochem 72:248–254. doi:10.1016/0003-2697(76)90527-3
Carr RB (1990) The fetal maternal placental unit. In: Becker KL (ed) Principles and practice of endocrinology and metabolism. J.B. Lippincott, pp 788
Casanueva E, Viteri FE (2003) Iron and oxidative stress in pregnancy. Am Soc Nutr Sci 133:1700S–1708S
Crichton RR, Wilmet S, Legssyer R, Ward RJ (2002) Molecular and cellular mechanisms of iron homeostasis and toxicity in mammalian cells. J Inorg Biochem 91:9–18. doi:10.1016/S0162-0134(02)00461-0
Crocker IP, Lawson PN, Baker PN, Fletcher J (2001) The anti-inflammatory effects of circulating fatty acids in obstructive jaundice: similarities with pregnancy-induced immunosuppression. QJM 94:475–484. doi:10.1093/qjmed/94.9.475
Dealtry GB, O’Farrell MK, Fernandez N (2000) The Th2 cytokine environment of the placenta. Int Arch Allergy Immunol 123(2):107–119. doi:10.1159/000024441
Dechend R, Viedt C, Muller DN, Ugele B, Brandes RP, Wallukat G et al (2003) AT1 receptor agonistic antibodies from preeclamptic patients stimulate NADPH oxidase. Circulation 107:1632–1639. doi:10.1161/01.CIR.0000058200.90059.B1
De Domenico I, Ward DM, Langelier C, Vaughn MB, Nemeth E, Sundquist WI et al (2007) The molecular mechanism of hepcidin-mediated ferroportin down-regulation. Mol Biol Cell 18:2569–2578. doi:10.1091/mbc.E07-01-0060
Dlaska M, Weiss G (1999) Central control of transcription factor NF-IL6 for cytokine and iron mediated regulation of murine inducible nitric oxide synthase expression. J Immunol 162:6171–6177
Dupic F, Fruchon S, Bensaid M, Loreal O, Brissot P, Borot N et al (2002) Duodenal mRNA expression of iron related genes in response to iron loading and iron deficiency in four strains of mice. Gut 51:648–653. doi:10.1136/gut.51.5.648
Ibouno LE, Shu EN, Igbokwe GE (1996) An improved technique for the assay of red blood cell superoxide dismutase (SOD) activity. Clin Chim Acta 247:1–6. doi:10.1016/0009-8981(95)06193-2
Kim S, Ponka P (2000) Effects of interferon-γ and lipopolysaccharide on macrophage iron metabolism are mediated by nitric oxide-induced degradation of iron regulatory protein 2. J Biol Chem 275:6220–6226. doi:10.1074/jbc.275.9.6220
Kustermans G, El Benna J, Piette J, Legrand-Poels S (2005) Perturbation of actin dynamics induces NF-kappaB activation in myelomonocytic cells through an NADPH oxidase-dependent pathway. Biochem J 387:531–540. doi:10.1042/BJ20041318
Lachili B, Hininger I, Faure H, Arnaud J, Richard MJ, Favier A et al (2001) Increased lipid peroxidation in pregnant women after iron and vitamin C supplementation. Biol Trace Elem Res 83:103–110. doi:10.1385/BTER:83:2:103
Legssyer R, Ward RJ, Crichton RR (1999) Effect of chronic chloroquine administration on iron loading in the liver and reticuloendothelial system and on oxidative responses by the macrophage. Biochem Pharmacol 57:907–911. doi:10.1016/S0006-2952(98)00368-2
Legssyer R, Josse C, Piette J, Ward RJ, Crichton RR (2003) Changes in function of iron-loaded alveolar macrophages after in vivo administration of desferrioxamine and/or chloroquine. J Inorg Biochem 94:36–42. doi:10.1016/S0162-0134(02)00633-5
Lu DY, Liou HC, Tang CH, Fu WM (2006) Hypoxia-induced iNOS expression in microglia is regulated by the PI3-kinase/Akt/mTOR signaling pathway and activation of hypoxia inducible factor-1alpha. Biochem Pharmacol 72:992–1000. doi:10.1016/j.bcp. 2006.06.038
Mellor A, Munn DH (2000) Immunology at the maternal-fetal interface: lessons for T cell tolerance and suppression. Annu Rev Immunol 18:367–391. doi:10.1146/annurev.immunol.18.1.367
Millard K, Frazer D, Wilkins SJ, Anderson GR (2004) Changes in the expression of intestinal iron transport and hepatic regulatory molecules explain the enhanced iron absorption associated with pregnancy in the rat. Gut 53:655–660. doi:10.1136/gut.2003.031153
Mukhopadhyay A, Bhatla N, Kriplani A, Agarway N, Saxena R (2004) Erythrocyte indices in pregnancy: effect of intermittent iron supplementation. Natl Med J India 17:135–137
Myatt L, Cui X (2004) Oxidative stress in the placenta. Histochem Cell Biol 122:369–382. doi:10.1007/s00418-004-0677-x
Nemeth E, Ganz T (2006) Regulation of iron metabolism by hepcidin. Annu Rev Nutr 26:323–342. doi:10.1146/annurev.nutr.26.061505.111303
Nemeth E, Tuttle MS, Powelson J, Vaughn MB, Donovan A, Ward DM et al (2004) Hepcidin regulates cellular iron efflux by binding to ferroportin and inducing its internalization. Science 306:2090–2093. doi:10.1126/science.1104742
Oppenheimer SJ (2001) Iron and its relation to immunity and infectious disease. J Nutr 131:616S–635S
Park CH, Valore EV, Waring AJ, Ganz T (2001) Hepcidin, a urinary antimicrobial peptide synthesized in the liver. J Biol Chem 276:7806–7810. doi:10.1074/jbc.M008922200
Qanungo S, Mukherjea M (2000) Ontogenic profile of some antioxidants and lipid peroxidation in human placental and fetal tissues. Mol Cell Biochem 215:11–19. doi:10.1023/A:1026511420505
Raghupathy R (1997) Th1-type immunity is incompatible with successful pregnancy. Immunol Today 18(10):478–482. doi:10.1016/S0167-5699(97)01127-4
Rioux FM, LeBlanc CP (2007) Iron supplementation during pregnancy: what are the risks and benefits of current practices? Appl Physiol Nutr Metab 32:282–288. doi:10.1139/H07-012
Stolzfus R, Dreyfuss M (1988) Guidelines for the use of iron supplements to prevent and treat iron deficiency anaemia. International nutritional anaemia consultative group. ILSI Press, Washington
Thomas C, Oates PS (2002) IEC-6 cells are an appropriate model of intestinal iron absorption in rats. J Nutr 132:680–687
Uotila J, Tuimala R, Aarnio T, Pyykko K, Ahotupa M (1991) Lipid peroxidation products, selenium-dependent glutathione peroxidase and vitamin E in normal pregnancy Eur J Obstet. Reprod Biol 42:95–100
Ward RJ, Kuhn L, Kaldy P, Florence A, Peters TJ, Crichton RR (1994) Control of cellular iron homeostasis by Iron Responsive Element (IRE) in vivo. Eur J Biochem 220:927–931. doi:10.1111/j.1432-1033.1994.tb18696.x
Ward RJ, Zhang Y, Crichton RR, Biret B, Piette J, De Witte P (1996) Expression of NFKappaB in rat brain after administration of ethanol in vivo. FEBS Lett 389:119–122. doi:10.1016/0014-5793(96)00545-5
Ward RJ, Wilmet S, Legssyer R, Crichton RR (2004) Iron supplementation during pregnancy-a necessary or toxic supplement. Bioinorg Chem Appl 1:169–176. doi:10.1155/S156536330300013X
Wisdom S, Wilson R, McKillop JH, Walker JJ (1991) Antioxidant systems in normal pregnancy and in pregnancy-induced hypertension. Am J Obstet Gynecol 165:170–174
WHO (2001) WHO/NHD/01.3. Geneva
Zhang Y, Crichton RR, Boelaert JR, Jorens PG, Herman AG, Ward RJ et al (1998) Decreased release of nitric oxide by alveolar macrophages after in vivo loading with iron or ethanol. Biochem Pharmacol 55:21–25. doi:10.1016/S0006-2952(97)00382-1
Acknowledgements
This work was supported by EC grant QLK1-CT-1999-00337. We are grateful to Vifor Pharmaceuticals for the supply of iron dextran and to Dr. N. Beaumont for advice with the RT-PCR assays.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ward, R.J., Wilmet, S., Legssyer, R. et al. Effects of marginal iron overload on iron homeostasis and immune function in alveolar macrophages isolated from pregnant and normal rats. Biometals 22, 211–223 (2009). https://doi.org/10.1007/s10534-008-9155-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10534-008-9155-6