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Forced expression of CD200 improves the differentiation capability and immunoregulatory functions of mesenchymal stromal cells

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Abstract

Objective

In order to identify specific mesenchymal stromal cell (MSC) populations with enhanced therapeutic efficacy, we evaluated the functional changes associated with the stable expression of CD200, which is associated with immune regulatory function and osteogenic differentiation, in human bone marrow-derived MSCs (CD200/MSCs).

Results

We detected significantly greater osteogenesis and chondrogenesis in CD200/MSCs than in mock-transfected MSCs. In addition, the immune regulatory function of MSCs in mixed lymphocyte reactions was enhanced by CD200 gene transfection. In CD200/MSCs, the secretion of inflammatory cytokines, i.e., IL-6 and IL-8, was reduced, and levels of the anti-inflammatory factors IL-10, FOXP3, and indoleamine 2,3-dioxygenase 1 were elevated. Finally, CD200 transfection increased the stemness of MSCs, as evidenced by greater colony numbers in colony-forming unit fibroblast assays and analyses of NANOG and OCT-4 expression.

Conclusions

These results suggest that CD200/MSCs have therapeutic applications, and further in-depth research should focus on the development of a clinically applicable cell-based therapeutic strategy.

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Acknowledgements

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Science, ICT & Future Planning (Grant Nos. 2015R1A2A2A04002756 and 2018R1A2B2006820). It was also supported by the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (Grant Nos. 2010-0008762, 2014R1A1A3054664).

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Author notes

  1. Hye Joung Kim and Kyoung-Woon Kim have contributed equally to this work.

Authors and Affiliations

  1. Laboratory of Hematological Disease and Transplant Immunology, Seoul, Korea

    Hye Joung Kim, Yong-Rim Kwon & Yoo-Jin Kim

  2. Department of Hematology, Convergent Research Consortium for Immunologic Disease, Seoul, Korea

    Hye Joung Kim, Kyoung-Woon Kim, Yong-Rim Kwon, Bo-Mi Kim & Yoo-Jin Kim

  3. Catholic Blood and Marrow Transplantation Center, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, 222 Banpo-Daero, Seocho-Gu, Seoul, 06591, Korea

    Yoo-Jin Kim

  4. Leukemia Research Institute, College of Medicine, The Catholic University of Korea, Seoul, Korea

    Yoo-Jin Kim

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  1. Hye Joung Kim
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  2. Kyoung-Woon Kim
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  3. Yong-Rim Kwon
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Correspondence to Yoo-Jin Kim.

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The authors declare that they have no conflict of interest.

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Kim, H.J., Kim, KW., Kwon, YR. et al. Forced expression of CD200 improves the differentiation capability and immunoregulatory functions of mesenchymal stromal cells. Biotechnol Lett 40, 1425–1433 (2018). https://doi.org/10.1007/s10529-018-2561-0

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  • DOI: https://doi.org/10.1007/s10529-018-2561-0

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